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Dears,
I download the genome (fasta) and its annotation (GFF) of an untypical organism from JGI (Joint GenomeInstitute) database, and want to set them as reference for RNAseq analysis using the star + RSEM strategy. On my macOS, the command was as below:
rsem-prepare-reference --gff3 /ref/Aurli1_GeneCatalog_genes_20120618.gff \ --star \ --star-path /Users/xinjun/miniconda3/envs/rnaseq/bin/star \ /ref/Aurli1_AssemblyScaffolds.fasta \ /Auran_RSEM/auran_rsem
However, an error message “Invalid number of arguments!” always occurs. How to resolve this? Thanks so much.
Xinjun
The text was updated successfully, but these errors were encountered:
Dears,
I download the genome (fasta) and its annotation (GFF) of an untypical organism from JGI (Joint GenomeInstitute) database, and want to set them as reference for RNAseq analysis using the star + RSEM strategy. On my macOS, the command was as below:
rsem-prepare-reference --gff3 /ref/Aurli1_GeneCatalog_genes_20120618.gff \ --star \ --star-path /Users/xinjun/miniconda3/envs/rnaseq/bin/star \ /ref/Aurli1_AssemblyScaffolds.fasta \ /Auran_RSEM/auran_rsem
However, an error message “Invalid number of arguments!” always occurs. How to resolve this? Thanks so much.
Xinjun
The text was updated successfully, but these errors were encountered: