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Hi,
I am a user of RSEM software and encountered some problems during use. The quantitative result of RSEM I got was 0, that is, most of the transcripts had no expression. However, for the bam files, I got quantitative results via StringTie calculations, which suggests that there may be no problem with the STAR software-based alignment process. I suspect that it is caused by the gtf file, because I am using the annotation given by the gffcompare software, which lacks the feature entry of gene, but I am not sure and hope to confirm it with you.
Thanks
The text was updated successfully, but these errors were encountered:
Hi,
I am a user of RSEM software and encountered some problems during use. The quantitative result of RSEM I got was 0, that is, most of the transcripts had no expression. However, for the bam files, I got quantitative results via StringTie calculations, which suggests that there may be no problem with the STAR software-based alignment process. I suspect that it is caused by the gtf file, because I am using the annotation given by the gffcompare software, which lacks the feature entry of gene, but I am not sure and hope to confirm it with you.
Thanks
The text was updated successfully, but these errors were encountered: