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In the following, PR #335@jspaezp introduced the possibility to perform the DIA without the preanalysis step. It would be great to use the SDRF information to sub-select a few RAW files to perform the preanalysis which is better to generate the final results. Some of the SDRF columns that could be selected to generate the preanalysis are:
factor value: We can randomly select files for each factor value.
technical and biological replicates: We should not pre-analyse biological and technical replicates.
The text was updated successfully, but these errors were encountered:
As @jspaezp mentioned, we should refine the current proposal for skipping the assembly library and pre-analysis using the following logic:
One variable called random_preliminary_analysis will control how many files will be used for random selection of files.
Another variable empirical_assembly_sample_n should control the number of variables needed to randomly perform the preanalysis.
This will help us to perform the pre-analysis on a certain number of files only depending on the size of the cluster and the resources, by making it false, it will use always all the files. For really large datasets users can define the number of files they want to use.
My main questions is how the previous PR #335 by @jspaezp overlaps with this idea. In the previous PR the user needs to do job1 and job2 configurations manually?
Description of feature
In the following, PR #335 @jspaezp introduced the possibility to perform the DIA without the preanalysis step. It would be great to use the SDRF information to sub-select a few RAW files to perform the preanalysis which is better to generate the final results. Some of the SDRF columns that could be selected to generate the preanalysis are:
The text was updated successfully, but these errors were encountered: