Adding essential parameter to primer3 Config file

nikolamueller · web-flow · commit 5eb1ba32c870 · 2020-07-10T17:23:38.000+02:00
The bartseq protocol requires an A at 3' primer position. There is a parameter to make this happen directly in primer3.
diff --git a/barcode_primer_design/example-input-data/PrimerSelect Example Configuration File.txt b/barcode_primer_design/example-input-data/PrimerSelect Example Configuration File.txt
@@ -116,6 +116,7 @@ PRIMER_SEQUENCING_INTERVAL=250
 PRIMER_SEQUENCING_LEAD=50
 PRIMER_SEQUENCING_SPACING=500
 PRIMER_TASK=generic
+PRIMER_MUST_MATCH_THREE_PRIME=nnnna
 PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
 PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
 PRIMER_TM_FORMULA=1
