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d4straub committed Nov 15, 2024
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Expand Up @@ -214,19 +214,19 @@ Please note the following additional requirements:

Taxonomic classification of ASVs can be performed with tools DADA2, SINTAX, Kraken2 or QIIME2. Multiple taxonomic reference databases are pre-configured for those tools, but user supplied databases are also supported for some tools. Alternatively (or in addition), phylogenetic placement can be used to extract taxonomic classifications.

In case multiple tools for taxonomic classification are executed in one pipeline run, only the taxonomic classification result of one tool is forwarded to downstream analysis with QIIME2. The priority is `phylogenetic placement` > `DADA2` > `SINTAX` > `Kraken2` > `QIIME2`.
In case multiple tools for taxonomic classification are executed in one pipeline run, only the taxonomic classification result of one tool is forwarded to downstream analysis with QIIME2. The priority is `phylogenetic placement` > `DADA2` > `SINTAX` > `Kraken2` > `QIIME2`, that is by no means a recommendation for a specific tool but a technical limitation.

Default setting for taxonomic classification is DADA2 with the SILVA reference taxonomy database.

Pre-configured reference taxonomy databases are:

| Database key | DADA2 | SINTAX | Kraken2 | QIIME2 | Target genes |
| ------------ | ----- | ------ | ------- | ------ | --------------------------------------------- |
| silva | + | - | + | + | 16S rRNA |
| gtdb | +¹ | - | - | - | 16S rRNA |
| silva | +¹ | - | + | + | 16S rRNA |
| gtdb | +² | - | - | - | 16S rRNA |
| sbdi-gtdb | + | - | - | - | 16S rRNA |
| rdp | + | - | + | - | 16S rRNA |
| greengenes | - | - | + | (+)² | 16S rRNA |
| greengenes | - | - | + | (+)³ | 16S rRNA |
| greengenes2 | - | - | - | + | 16S rRNA |
| pr2 | + | - | - | - | 18S rRNA |
| unite-fungi | + | + | - | - | eukaryotic nuclear ribosomal ITS region |
Expand All @@ -235,9 +235,9 @@ Pre-configured reference taxonomy databases are:
| midori2-co1 | + | - | - | - | eukaryotic Cytochrome Oxidase I (COI) |
| phytoref | + | - | - | - | eukaryotic plastid 16S rRNA |
| zehr-nifh | + | - | - | - | Nitrogenase iron protein NifH |
| standard | - | - | + | - | any in genomes of archaea, bacteria, viruses³ |
| standard | - | - | + | - | any in genomes of archaea, bacteria, viruses |

¹[`--dada_taxonomy_rc`](https://nf-co.re/ampliseq/parameters#dada_taxonomy_rc) is recommended; ²: de-replicated at 85%, only for testing purposes; ³: quality of results might vary
¹: As of Silva version 138 optimized for classification of Bacteria and Archaea, not suitable for Eukaryotes; ²[`--dada_taxonomy_rc`](https://nf-co.re/ampliseq/parameters#dada_taxonomy_rc) is recommended; ³: de-replicated at 85%, only for testing purposes; : quality of results might vary

Special features of taxonomic classification tools:

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