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Decide on reasonable min-length filter #33

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j23414 opened this issue Oct 18, 2024 · 1 comment
Open

Decide on reasonable min-length filter #33

j23414 opened this issue Oct 18, 2024 · 1 comment
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enhancement New feature or request

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@j23414
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j23414 commented Oct 18, 2024

Context

Noticed some WA samples were being dropped due to the min-length filter (originally set to include at least 90% of the genome or 9800nt). We're considering dropping that lower to include more sequences as long as the phylogenetic tree looks reasonable.

The augur index of the WA sequences: wa_stats.txt

@j23414 j23414 added the enhancement New feature or request label Oct 18, 2024
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j23414 commented Oct 25, 2024

Just documenting that min-length can be adjusted in the following locations:

Washington-specific

subsampling:
state: --query "state == 'WA'" --min-length '9800' --subsample-max-sequences 5000
neighboring_state: --query "state in ['CA', 'ID', 'OR', 'NV']" --group-by state year --min-length '9800' --subsample-max-sequences 5000
region: --query "state in ['AZ','NM', 'CO', 'UT', 'WY', 'MT']" --group-by state year --min-length '9800' --subsample-max-sequences 5000
country: --query "country == 'USA' and state not in ['WA', 'CA', 'ID', 'OR', 'NV','AZ','NM', 'CO', 'UT', 'WY', 'MT'] and accession != 'NC_009942'" --group-by state year --subsample-max-sequences 300 --min-length '9800'
force_include: --exclude-all --include ../nextclade/defaults/include.txt

global

subsampling:
region: --query "is_lab_host != 'true'" --query-columns is_lab_host:str --min-length '9800' --group-by region year --subsample-max-sequences 3000 --exclude defaults/exclude.txt
force_include: --exclude-all --include defaults/include.txt

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