@@ -21,7 +21,7 @@ cd minimap2 && make
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./minimap2 -ax splice -uf -k14 ref.fa reads.fa > aln.sam # noisy Nanopore direct RNA-seq
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./minimap2 -ax splice:hq -uf ref.fa query.fa > aln.sam # PacBio Kinnex/Iso-seq (RNA-seq)
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./minimap2 -ax splice --junc-bed anno.bed12 ref.fa query.fa > aln.sam # use annotated junctions
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- ./minimap2 -ax splice:sr ref.fa r1.fq r2.fq > aln.sam # short-read RNA-seq (r1236 or later )
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+ ./minimap2 -ax splice:sr ref.fa r1.fq r2.fq > aln.sam # short-read RNA-seq (r1236+; experimental )
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./minimap2 -cx asm5 asm1.fa asm2.fa > aln.paf # intra-species asm-to-asm alignment
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./minimap2 -x ava-pb reads.fa reads.fa > overlaps.paf # PacBio read overlap
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./minimap2 -x ava-ont reads.fa reads.fa > overlaps.paf # Nanopore read overlap
@@ -40,7 +40,7 @@ man ./minimap2.1
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- [ Map long mRNA/cDNA reads] ( #map-long-splice )
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- [ Find overlaps between long reads] ( #long-overlap )
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- [ Map short genomic reads] ( #short-genomic )
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- - [ Map short RNA-seq reads] ( #short-rna-seq )
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+ - [ Map short RNA-seq reads (experimental & evolving) ] ( #short-rna-seq )
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- [ Full genome/assembly alignment] ( #full-genome )
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- [ Advanced features] ( #advanced )
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- [ Working with >65535 CIGAR operations] ( #long-cigar )
@@ -231,14 +231,16 @@ be paired if they are adjacent in the input stream and have the same name (with
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the ` /[0-9] ` suffix trimmed if present). Single- and paired-end reads can be
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mixed.
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- #### <a name =" short-rna-seq " ></a >Map short RNA-seq reads
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+ #### <a name =" short-rna-seq " ></a >Map short RNA-seq reads (experimental & evolving)
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``` sh
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minimap2 -ax splice:sr ref.fa reads-se.fq > aln.sam # single-end
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minimap2 -ax splice:sr ref.fa r1.fq r2.fq > aln.sam # paired-end
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```
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The new preset ` splice:sr ` was added between v2.28 and v2.29. It functions
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- similarly to ` sr ` except that it performs spliced alignment.
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+ similarly to ` sr ` except that it performs spliced alignment. Note that this
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+ functionality is *** experiemental*** and evolving. It is better not to use it
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+ for production.
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#### <a name =" full-genome " ></a >Full genome/assembly alignment
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