Track Spots (Subpixel localization) Not Working Cause: null

[Guoming-Gao]

Hi, I have had no luck debugging this for several weeks now. I tried both Linux and Mac versions of KNIME, tried different versions of KNIME, and tried different releases of this. There is still the same error.
The full error message is :
WARN Track Spots (Subpixel localization) 0:428 Error while executing row: xxxxx.tif! Cause: null

xxxxx would be the name of my video, and the 0:428 seems to be a random number. It's consistent in the same version of KNIME, but will change if the same workflow was run on different versions of KNIME. The output of the Track Spots node will be a lot of '?' in each array element.

Any help will be greatly appreciated!!

[Guoming-Gao]

When run on Mac, there's an additional error message

ERROR ThreadPool An exception occurred while executing a runnable.

[imagejan]

Sorry for the delay on this one.
Unfortunately, the error logging within the KNIME ImageJ integration is not too helpful for this kind of errors. I've seen similar errors in the past when the detection/tracking parameters were unsuitable for a given image, or when there were mismatches between image calibration (i.e. pixel spacing) and spot detection parameters (radius).

@Guoming-Gao if you still have this issue, would you mind sharing a minimal workflow to reproduce it?