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Update 2022-06-21-Svalbard-expedition-2022-report.md
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jacopaso authored May 3, 2024
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Expand Up @@ -36,3 +36,5 @@ Once the sampling campaign has ended, the samples are taken to the laboratory wh
As for the molecular analysis, we will extract DNA and amplify a conserved genomic region through a PCR, which is a Polymerase Chain Reaction. This last step is important to make numerous copies of just one piece of DNA, whose sequence can reveal the phylogenetic relationship of a microorganism to a specific taxonomic group. The resulting data will be used to perform both phylogenetic and meta-genomic analyses, enabling us to take a look at the most abundant microbial groups but also at the main functions and metabolisms characterizing each sample.

On the same samples, geochemical analysis will be performed through special instruments such as the Elemental Analyzer and the ICP-MS (Inductively Coupled Plasma Mass Spectrometer). In particular we will evaluate the total carbon, nitrogen and sulfur (major elements) using an Elemental Analyzer, and the trace elements concentration with the ICP-MS. This data will allow us to evaluate the depth, as well as various correlating organic or inorganic sediment compounds, as important drivers for microbial diversity in this environment.

Fotos: Jacopo Pasotti, 2023

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