Scripts for "Constitutive activation of cellular immunity underlies the evolution of resistance to infection in Drosophila"
Alexandre B Leitão*, Ramesh Arunkumar*, Jonathan P Day,Emma M Geldman,Ismaël Morin-Poulard, Michèle Crozatier, Francis M Jiggins https://doi.org/10.7554/eLife.59095
Goal was to identify cell types in Drosophila larval hemocytes (blood cells) from 10X single cell RNA-sequencing using Seurat
singlecell_dmel_hemocytes.R - Single cell analysis
DoHeatmap2.R - Modified Seurat v3 DoHeatmap function for plotting
compare_datasets.R - Comparing three Drosophilia single cell hemocyte datasets
app.R - Shiny app for single cell gene expression browser
also in https://doi.org/10.7554/eLife.59095
https://arunkuma.shinyapps.io/singlecelldmel/
![Screenshot 2024-01-25 at 16 44 31](https://private-user-images.githubusercontent.com/23363383/299722912-9233d571-6fd4-4da0-a657-b07c85b3c908.png?jwt=eyJhbGciOiJIUzI1NiIsInR5cCI6IkpXVCJ9.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.PCu9De6-qlgtpF2LvpgtxIWRbf4FPwhSbRMZPgAwbVA)