This repository supports the development of the first published genome sequence of the popular turfgrass, St. Augustinegrass. This reference genome provides a high-quality closely related genome for future genomic work with warm-season turfgrasses and will allow for the expansion of the molecular toolkits that are available to turfgrass breeders to allow building tools to enable more efficient selection.
- How to cite - Will be updated upon publication.
This is a project supported by the U.S. Department of Agriculture - Agricultural Research Service (USDA-ARS)
Genomics and Bioinformatics Research Unit (GBRU).
We present a reference-grade chromosome-scale genome assembly for the popular freeze-tolerant diploid cultivar ‘Raleigh’, utilizing data from PacBio CCS, Illumina, and Hi-C technologies. The reference genome has been resolved into two haplotype assemblies (465.41 and 401.52 Mb), accounting for 95.2% and 82.1% of the expected haplotype genome size respectively, each anchored on the nine chromosomes and a total of 62,454 genes. Analysis of the assembly revealed 50.70% of the genome contained repeats. Analysis of the diversity within the species not covered by the reference genome was obtained by a panel of 79 genotypes including commercial cultivars, breeding lines, and plant introductions by low-coverage sequencing identifying 605,038 single nucleotide polymorphisms (SNPs).
- Includes all files related to analyzing low coverage riptide sequencing data and performing diversity analysis with cultivar materials. Raw sequences for all samples has been deposited in NCBI under BioProject PRJNA1007876.
- All files related to generating the gene annotation for the genome assembly can be found in this additional github repo.
This is a project supported by the U.S. Department of Agriculture - Agricultural Research Service (USDA-ARS) - Genomics and Bioinformatics Research Unit (GBRU) through CRIS Project No. 6066-21310-006-000-D. Additional project support for riptide sequencing was through Breeding Insight, funded by USDA-ARS.