Microbial Composition Analysis Tool

Overview

The Microbial Composition Analysis Tool is a Python-based image analysis tool designed for quantifying and analyzing microbial composition in fluorescence microscopy images obtained through FISH (Fluorescence In Situ Hybridization) experiments.

Features

• Quantification of different microbial color categories (e.g., purple, blue, green) in fluorescence microscopy images.
• Color categories are defined based on RGB parameters (e.g. ratios between colors).
• Calculation of percentages for each color category to provide insights into microbial distribution.
• Compatibility with images obtained through FISH procedures using specific probes.

Getting Started

Prerequisites

• Python 3.x
• Python Imaging Library (PIL)
• File 'Image processing FISH.ipynb' contains the required functions to quantify the FISH images.