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Abr update liquid setups (#16825)
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# Overview
Update to liquid setup protocols for abr use

---------

Co-authored-by: rclarke0 <[email protected]>
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AnthonyNASC20 and rclarke0 authored Nov 14, 2024
1 parent 3cd7c2f commit a355384
Showing 1 changed file with 45 additions and 5 deletions.
Original file line number Diff line number Diff line change
Expand Up @@ -35,15 +35,23 @@ def run(protocol: protocol_api.ProtocolContext) -> None:
res1 = protocol.load_labware("nest_12_reservoir_15ml", "D2", "reagent reservoir 1")
# Label Reservoirs
well1 = res1["A1"].top()
well2 = res1["A2"].top()
well3 = res1["A3"].top()
well4 = res1["A4"].top()
well5 = res1["A5"].top()
well6 = res1["A6"].top()
well7 = res1["A7"].top()
well8 = res1["A8"].top()
well9 = res1["A9"].top()
well10 = res1["A10"].top()

well11 = res1["A11"].top()
well12 = res1["A12"].top()
# Volumes
wash = 600
al_and_pk = 468
beads_and_binding = 552
binding = 320
beads = 230
pk = 230
lysis = 230

# Sample Plate
p1000.transfer(
Expand All @@ -65,9 +73,41 @@ def run(protocol: protocol_api.ProtocolContext) -> None:
)
# Res 1
p1000.transfer(
volume=[beads_and_binding, al_and_pk, wash, wash, wash],
volume=[
binding,
beads,
binding,
beads,
lysis,
pk,
wash,
wash,
wash,
wash,
wash,
wash,
wash,
wash,
wash,
],
source=source_reservoir["A1"].bottom(z=0.5),
dest=[well1, well3, well4, well7, well10],
dest=[
well1,
well1,
well2,
well2,
well3,
well3,
well4,
well5,
well6,
well7,
well8,
well9,
well10,
well11,
well12,
],
blowout=True,
blowout_location="source well",
trash=False,
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