chore(Release): 2024-02-21

protoBuilds/274d2a/README.json

@@ -1,5 +1,5 @@
 {
-    "author": "Opentrons\nOpentrons has launched a new Protocol Library. You should use the new page for this protocol. This page won\u2019t be available after March 31st, 2024.",
+    "author": "Opentrons",
     "categories": {
         "Sample Prep": [
             "Cherrypicking"
@@ -8,7 +8,7 @@
     "description": "\nCherrypicking, or hit-picking, is a key component of many workflows from high-throughput screening to microbial transfections. With this protocol, you can easily select specific wells in any labware without worrying about missing or selecting the wrong well. Just upload your properly formatted CSV file (keep scrolling for an example), customize your parameters, and download your ready-to-run protocol.\n\n\nTo purchase tips, reagents, or pipettes, please visit our online store or contact our sales team at [email protected]\n\nOpentrons OT-2\nOpentrons OT-2 Run App (Version 3.15.0 or later)\nOpentrons Single-Channel Pipette and corresponding Tips\nMicroplates (96-well or 384-well)\n\nFor more detailed information on compatible labware, please visit our Labware Library.\n\n\nLabware will be loaded automatically by specifying the labware loadname and labware slot in the .csv file. All available empty slots will be filled with the necessary tipracks, and the user will be prompted to refill the tipracks if all are emptied in the middle of the protocol.\nCSV Format\nYour cherrypicking transfers must be saved as a comma separated value (.csv) file type. Your CSV must contain values corresponding to volumes in microliters (\u03bcL). Note that the header line (first row of the .csv file) should also be included!\nHere's an example of how a short cherrypicking protocol should be properly formatted:\nNew Tip,Source Labware,Source Slot,Source Well,Source Aspiration Height Above Bottom (in mm),Dest Labware,Dest Slot,Dest Well,Dest Dispense Height Above Bottom (in mm),Volume (in ul)\nyes,agilent_1_reservoir_290ml,1,A1,1,nest_96_wellplate_100ul_pcr_full_skirt,4,A11,2,1\n,nest_12_reservoir_15ml,2,A1,1,corning_384_wellplate_112ul_flat,5,B11,2,3\nyes,nest_1_reservoir_195ml,3,A1,1,corning_384_wellplate_112ul_flat,5,A12,2,7\nIn the above example, 1\u03bcL will be transferred from 1mm above the bottom of well A1 in an Agilent 1-well 290ml reservoir (slot 1) to well A11 in the destination NEST 96-well plate 100\u00b5l (slot 4) with a new tip. After this, 3\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 12-well 15ml reservoir (slot 2) to well A5 in the destination NEST 96-well plate 100\u00b5l (slot 5) with the same tip. Last, 7\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 1-well 195ml reservoir (slot 3) to well H12 in the destination NEST 96-well plate 100\u00b5l (slot 5) with a new tip.\nIf you\u2019d like to follow our template, you can make a copy of this spreadsheet, fill out your values, and export as CSV for use with this protocol.\nUsing the customizations fields, below set up your protocol.\n Transfer .csv File: Upload the .csv file containing your well locations, volumes, and source plate (optional).\n Pipette Model: Select which pipette you will use for this protocol.\n Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n Tip Type: Specify whether you want to use filter tips.\n* Tip Usage Strategy: Specify whether you'd like to use a new tip for each transfer, or keep the same tip throughout the protocol.",
     "internal": "274d2a",
     "markdown": {
-        "author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/274d2a). This page won\u2019t be available after March 31st, 2024.\n\n",
+        "author": "[Opentrons](https://opentrons.com/)\n\n\n",
         "categories": "* Sample Prep\n\t* Cherrypicking\n\n",
         "description": "![Cherrypicking Example](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/cherrypicking/cherrypicking_example.png)\n\nCherrypicking, or hit-picking, is a key component of many workflows from high-throughput screening to microbial transfections. With this protocol, you can easily select specific wells in any labware without worrying about missing or selecting the wrong well. Just upload your properly formatted CSV file (keep scrolling for an example), customize your parameters, and download your ready-to-run protocol.\n\n---\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/materials.png)\n\nTo purchase tips, reagents, or pipettes, please visit our [online store](https://shop.opentrons.com/) or contact our sales team at [[email protected]](mailto:[email protected])\n\n* [Opentrons OT-2](https://shop.opentrons.com/collections/ot-2-robot/products/ot-2)\n* [Opentrons OT-2 Run App (Version 3.15.0 or later)](https://opentrons.com/ot-app/)\n* [Opentrons Single-Channel Pipette](https://shop.opentrons.com/collections/ot-2-pipettes) and corresponding [Tips](https://shop.opentrons.com/collections/opentrons-tips)\n* [Microplates (96-well or 384-well)](https://labware.opentrons.com/?category=wellPlate)\n\nFor more detailed information on compatible labware, please visit our [Labware Library](https://labware.opentrons.com/).\n\n\n\n---\n![Setup](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/Setup.png)\n\nLabware will be loaded automatically by specifying the labware loadname and labware slot in the .csv file. All available empty slots will be filled with the necessary [tipracks](https://shop.opentrons.com/collections/opentrons-tips), and the user will be prompted to refill the tipracks if all are emptied in the middle of the protocol.\n\n**CSV Format**\n\nYour cherrypicking transfers must be saved as a comma separated value (.csv) file type. Your CSV must contain values corresponding to volumes in microliters (\u03bcL). Note that the header line (first row of the .csv file) should also be included!\n\nHere's an example of how a short cherrypicking protocol should be properly formatted:\n\n```\nNew Tip,Source Labware,Source Slot,Source Well,Source Aspiration Height Above Bottom (in mm),Dest Labware,Dest Slot,Dest Well,Dest Dispense Height Above Bottom (in mm),Volume (in ul)\nyes,agilent_1_reservoir_290ml,1,A1,1,nest_96_wellplate_100ul_pcr_full_skirt,4,A11,2,1\n,nest_12_reservoir_15ml,2,A1,1,corning_384_wellplate_112ul_flat,5,B11,2,3\nyes,nest_1_reservoir_195ml,3,A1,1,corning_384_wellplate_112ul_flat,5,A12,2,7\n```\n\nIn the above example, 1\u03bcL will be transferred from 1mm above the bottom of well A1 in an Agilent 1-well 290ml reservoir (slot 1) to well A11 in the destination NEST 96-well plate 100\u00b5l (slot 4) with a new tip. After this, 3\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 12-well 15ml reservoir (slot 2) to well A5 in the destination NEST 96-well plate 100\u00b5l (slot 5) with the same tip. Last, 7\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 1-well 195ml reservoir (slot 3) to well H12 in the destination NEST 96-well plate 100\u00b5l (slot 5) with a new tip.\n\nIf you\u2019d like to follow our template, you can make a copy of [this spreadsheet](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/274d2a/ex.csv), fill out your values, and export as CSV for use with this protocol.\n\nUsing the customizations fields, below set up your protocol.\n* Transfer .csv File: Upload the .csv file containing your well locations, volumes, and source plate (optional).\n* Pipette Model: Select which pipette you will use for this protocol.\n* Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n* Tip Type: Specify whether you want to use filter tips.\n* Tip Usage Strategy: Specify whether you'd like to use a new tip for each transfer, or keep the same tip throughout the protocol.\n\n\n",
         "internal": "274d2a\n",

protoBuilds/3359a5/README.json

@@ -1,5 +1,5 @@
 {
-    "author": "Opentrons\nOpentrons has launched a new Protocol Library. You should use the new page for this protocol. This page won\u2019t be available after March 31st, 2024.",
+    "author": "Opentrons",
     "categories": {
         "Featured": [
             "Cherrypicking"
@@ -10,7 +10,7 @@
     "internal": "3359a5",
     "labware": "\nAny verified labware found in our Labware Library\n",
     "markdown": {
-        "author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/3359a5). This page won\u2019t be available after March 31st, 2024.\n\n",
+        "author": "[Opentrons](https://opentrons.com/)\n\n\n",
         "categories": "* Featured\n\t* Cherrypicking\n\n",
         "deck-setup": "Example Deck Setup - this is variable depending on the .csv uploaded.\n![deck layout](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/3359a5/Screen+Shot+2021-04-29+at+2.45.06+PM.png)\n\n\n---\n\n",
         "description": "With this protocol, your robot can perform multiple well-to-well liquid transfers using a single-channel pipette by parsing through a user-defined .csv file. The protocol can use Opentrons GEN1 or GEN2 pipettes.\n\nThis particular cherrypicking protocol  allows you to specify the source plate labware and slot number, as well as the aspiration height above the bottom of the well (in mm). Appropriate tip racks will be placed in every slot that isn't already populated with a source or destination labware.\n\n\nExplanation of complex parameters below:\n* `Pipette Type`: Specify which single channel pipette you will be using for this protocol.\n* `input .csv file`: Here, you should upload a .csv file formatted in the [following way](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/3359a5/template.csv) being sure to include the header line. Refer to our [Labware Library](https://labware.opentrons.com/?category=wellPlate) to copy API names for labware to include in the `source_labware` and `destination_labware` columns of the .csv.\n\n---\n\n",