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protocol library banner test #5000

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protocol library banner test #5000

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2 changes: 1 addition & 1 deletion protoBuilds/nucleic_acid_purification_with_magnetic_beads/README.json
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"description": "With this protocol, you can perform high-quality nucleic acid purifications using magnetic beads and the Opentrons Magnetic Module. This protocol contains flexible parameters that you can customize for many different magnetic bead and nucleic acid types. Use this setup to rapidly iterate and optimize your magbead-based workflows!\nYou can use any magnetic beads you prefer with this protocol, but we have included some reagent recommendations in the Materials Needed section below to help you get started. For more detailed information on how to use this protocol, please see our Technical Note.\n\n\n\n-- Opentrons OT-2\n-- Opentrons Magnetic Module\n-- Opentrons OT-2 Run App (Version 3.1.2 or later)\n-- 200uL or 300 uL Tiprack (Opentrons tips suggested)\n-- 12-row automation-friendly trough\n-- BioRad HardShell 96-Well PCR Plate\n-- Magnetic Beads (Looking for a kit? We recommend trying Omega Bio-tek Mag-Bind\u00ae TotalPure NGS)\n-- Ethanol\n-- Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water)\n\n\n\nUsing the customization fields below, set up your protocol as follows:\n\nPipette: Specify your pipette. We recommend using a p50 or p300 multi- or single-channel.\nPipette Mount: Specify which mount (left or right) your pipette is on.\nSample number: Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette.\nSample volume: Specify the starting volume (in uL) of the input sample.\nBead Ratio: Customize the ratio of beads for left or right side size-selection of fragments. The default bead ratio is 1.8x the input sample volume.\nElution Volume: Specify the final volume (in uL) to elute the purified nucleic acid. The Opentrons MagDeck supports elution volumes above 10 \u00b5L.\nIncubation Time: Specify the amount of time (in minutes) that the bead solution and input sample interact.\nSettling Time: Specify the amount of time (in minutes) needed to pellet the beads. Higher volumes may require a longer settling time.\nDrying Time: Specify the drying time (in minutes) needed after wash steps.\n\n\n",
"internal": "Nucleic Acid Purification, v1",
"markdown": {
"author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](library.opentrons.com/p/nucleic_acid_purification_with_magnetic_beads). This page won\u2019t be available after January 31st, 2024.\n\n",
"author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/nucleic_acid_purification_with_magnetic_beads). This page won\u2019t be available after January 31st, 2024.\n\n",
"categories": "* Featured\n * Nucleic Acid Purification with Magnetic Beads (Universal)\n\n",
"description": "With this protocol, you can perform high-quality nucleic acid purifications using magnetic beads and the [Opentrons Magnetic Module](https://shop.opentrons.com/products/magdeck]). This protocol contains flexible parameters that you can customize for many different magnetic bead and nucleic acid types. Use this setup to rapidly iterate and optimize your magbead-based workflows!\n\nYou can use any magnetic beads you prefer with this protocol, but we have included some reagent recommendations in the **Materials Needed** section below to help you get started. For more detailed information on how to use this protocol, please see our [Technical Note](https://s3.amazonaws.com/opentrons-protocol-library-website/Technical+Notes/Nucleic+Acid+Purification+with+Magnetic+Module+OT2+Technical+Note.pdf).\n\n---\n\n---\n\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/customizable-serial-dilution/materials.png)\n\n-- [Opentrons OT-2](http://opentrons.com/ot-2)\n\n-- [Opentrons Magnetic Module](https://shop.opentrons.com/products/magdeck?_ga=2.171718441.823190023.1542396855-403439593.1535387376)\n\n-- [Opentrons OT-2 Run App (Version 3.1.2 or later)](http://opentrons.com/ot-app)\n\n-- 200uL or 300 uL Tiprack ([Opentrons tips suggested](https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-300ul-tips-racks-9-600-tips))\n\n-- [12-row automation-friendly trough](https://www.usascientific.com/12-channel-automation-reservoir.aspx)\n\n-- [BioRad HardShell 96-Well PCR Plate](http://www.bio-rad.com/en-us/sku/hsp9601-hard-shell-96-well-pcr-plates-low-profile-thin-wall-skirted-white-clear?ID=hsp9601)\n\n-- Magnetic Beads (Looking for a kit? We recommend trying [Omega Bio-tek Mag-Bind\u00ae TotalPure NGS](https://shop.opentrons.com/products/mag-bind-total-pure-ngs))\n\n-- Ethanol\n\n-- Elution Buffer (Typically 10 mM Tris pH 8.0, TE Buffer, or nuclease-free water)\n\n---\n\n---\n\n![Setup](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/Setup.png)\n\nUsing the customization fields below, set up your protocol as follows:\n\n * **Pipette:** Specify your pipette. We recommend using a p50 or p300 multi- or single-channel.\n * **Pipette Mount:** Specify which mount (left or right) your pipette is on.\n * **Sample number:** Customize the number of samples to run per protocol. A multiple of 8 is recommended when you are using a multichannel pipette.\n * **Sample volume:** Specify the starting volume (in uL) of the input sample.\n * **Bead Ratio:** Customize the ratio of beads for left or right side size-selection of fragments. *The default bead ratio is 1.8x the input sample volume.*\n * **Elution Volume:** Specify the final volume (in uL) to elute the purified nucleic acid. *The Opentrons MagDeck supports elution volumes above 10 \u00b5L.*\n * **Incubation Time:** Specify the amount of time (in minutes) that the bead solution and input sample interact.\n * **Settling Time:** Specify the amount of time (in minutes) needed to pellet the beads. *Higher volumes may require a longer settling time.*\n * **Drying Time:** Specify the drying time (in minutes) needed after wash steps.\n\n---\n\n---\n\n",
"internal": "Nucleic Acid Purification, v1\n\n",
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2 changes: 1 addition & 1 deletion protocols/nucleic_acid_purification_with_magnetic_beads/README.md
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### Author
[Opentrons](https://opentrons.com/)

# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](library.opentrons.com/p/nucleic_acid_purification_with_magnetic_beads). This page won’t be available after January 31st, 2024.
# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/nucleic_acid_purification_with_magnetic_beads). This page won’t be available after January 31st, 2024.

## Categories
* Featured
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