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fix(protocols/gsdx): add flashing option, fix liquid volume display #4884

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Sep 13, 2023
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1 change: 1 addition & 0 deletions data/data/fields.csv
Original file line number Diff line number Diff line change
Expand Up @@ -383,6 +383,7 @@ dna_well_plate_tmod,1
do_cool_samples,1
do_deactivate_tc,1
do_dnase_step,1
do_flash,1
do_mm_resusp_pause,1
do_plate_mm,1
do_premix,1
Expand Down
17 changes: 16 additions & 1 deletion protoBuilds/gsdx/bacgene.ot2.apiv2.py.json

Large diffs are not rendered by default.

24 changes: 18 additions & 6 deletions protocols/gsdx/bacgene.ot2.apiv2.py
Original file line number Diff line number Diff line change
Expand Up @@ -29,12 +29,19 @@

def run(ctx):

def flash_lights():
initial_status = ctx.rail_lights_on
for _ in range(19):
ctx.set_rail_lights(not ctx.rail_lights_on)
ctx.delay(seconds=0.25)
ctx.set_rail_lights(initial_status)

[num_samples_listeria, num_samples_salmonella,
num_samples_listeria_remaining, num_samples_salmonella_remaining,
sample_rack_type, salmonella_meat] = get_values( # noqa: F821
sample_rack_type, salmonella_meat, do_flash] = get_values( # noqa: F821
'num_samples_listeria', 'num_samples_salmonella',
'num_samples_listeria_remaining', 'num_samples_salmonella_remaining',
'sample_rack_type', 'salmonella_meat')
'sample_rack_type', 'salmonella_meat', 'do_flash')

# modules
tempdeck = ctx.load_module('temperature module gen2', '10')
Expand Down Expand Up @@ -190,11 +197,15 @@ def run(ctx):
num_cols_offset_samples_listeria*8+num_samples_salmonella]

# load liquids
vol_sample = 5.0

try:
if num_samples_listeria > 0:
positive_control_l.load_liquid(positive_control_l_liq, volume=10)
positive_control_l.load_liquid(
positive_control_l_liq, volume=vol_sample)
if num_samples_salmonella > 0:
positive_control_s.load_liquid(positive_control_s_liq, volume=10)
positive_control_s.load_liquid(
positive_control_s_liq, volume=vol_sample)
[well.load_liquid(samples_listeria_liq_sample,
volume=30/len(samples_single_listeria))
for well in samples_single_listeria]
Expand Down Expand Up @@ -635,8 +646,6 @@ def wick(pip, well, x_magnitude=0.5, z_offset=3.0):
ctx.delay(minutes=10, msg='Incubating for 10 minutes @ 95C.')
tempdeck.set_temperature(37)

vol_sample = 5.0

if DO_TRANSFER_SAMPLE_TO_PCR:
""" transfer samples to PCR plate """

Expand Down Expand Up @@ -713,3 +722,6 @@ def wick(pip, well, x_magnitude=0.5, z_offset=3.0):
drop(m20)

tempdeck.deactivate()

if do_flash:
flash_lights()
101 changes: 58 additions & 43 deletions protocols/gsdx/fields.json
Original file line number Diff line number Diff line change
@@ -1,44 +1,59 @@
[
{
"type": "int",
"label": "number of listeria wells to run (including +/- controls)",
"name": "num_samples_listeria",
"default": 48
},
{
"type": "int",
"label": "number of salmonella wells to run (including +/- controls)",
"name": "num_samples_salmonella",
"default": 48
},
{
"type": "int",
"label": "number of listeria wells in kit remaining",
"name": "num_samples_listeria_remaining",
"default": 96
},
{
"type": "int",
"label": "number of salmonella wells in kit remaining",
"name": "num_samples_salmonella_remaining",
"default": 96
},
{
"type": "dropDown",
"label": "sample rack format",
"name": "sample_rack_type",
"options": [
{"label": "KingFisher 96 Tube Rack with GoldStandard 1.1 mL", "value": "kingfisher_96_tuberack_1100ul"},
{"label": "BIOPlastics BV 96 Aluminum Block 200 µL", "value": "bioplasticsbv_96_aluminumblock_200ul"}
]
},
{
"type": "dropDown",
"label": "running salmonella meat",
"name": "salmonella_meat",
"options": [
{"label": "no", "value": false},
{"label": "yes", "value": true}
]
}
]
{
"type": "int",
"label": "number of listeria wells to run (including +/- controls)",
"name": "num_samples_listeria",
"default": 48
},
{
"type": "int",
"label": "number of salmonella wells to run (including +/- controls)",
"name": "num_samples_salmonella",
"default": 48
},
{
"type": "int",
"label": "number of listeria wells in kit remaining",
"name": "num_samples_listeria_remaining",
"default": 96
},
{
"type": "int",
"label": "number of salmonella wells in kit remaining",
"name": "num_samples_salmonella_remaining",
"default": 96
},
{
"type": "dropDown",
"label": "sample rack format",
"name": "sample_rack_type",
"options": [
{
"label": "KingFisher 96 Tube Rack with GoldStandard 1.1 mL",
"value": "kingfisher_96_tuberack_1100ul"
},
{
"label": "BIOPlastics BV 96 Aluminum Block 200 µL",
"value": "bioplasticsbv_96_aluminumblock_200ul"
}
]
},
{
"type": "dropDown",
"label": "running salmonella meat",
"name": "salmonella_meat",
"options": [
{ "label": "no", "value": false },
{ "label": "yes", "value": true }
]
},
{
"type": "dropDown",
"label": "flash on completion",
"name": "do_flash",
"options": [
{ "label": "no", "value": false },
{ "label": "yes", "value": true }
]
}
]