fix

protoBuilds/4a0be6/4a0be6.ot2.apiv2.py.json

@@ -1,5 +1,5 @@
 {
-    "content": "import math\nfrom opentrons.protocol_api.labware import Well\n\nfrom opentrons import APIVersion\n\nmetadata = {\n    'protocolName': 'Tube Filling',\n    'author': 'Opentrons <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(protocol):\n    [transferVol, tubeRacks, tubeType, numRacks, srcType, srcVol,\n     pipType, pipMnt, startTip, dispMode, touchTip] = get_values(  # noqa: F821\n     'transferVol', 'tubeRacks', 'tubeType', 'numRacks', 'srcType', 'srcVol',\n     'pipType', 'pipMnt', 'startTip', 'dispMode', 'touchTip')\n\n    # Load labware and pipettes\n\n    tipNum = pipType.split('_')[0][1:]\n    tipNum = '300' if tipNum == '50' else tipNum\n    tipType = f'opentrons_96_tiprack_{tipNum}ul'\n    tips = [protocol.load_labware(tipType, '11')]\n    pip = protocol.load_instrument(pipType, pipMnt, tip_racks=tips)\n\n    firstTip = startTip[0].upper()+str(int(startTip[1:]))\n    pip.starting_tip = tips[0][firstTip]\n\n    srcLabware = protocol.load_labware(srcType, '1')\n\n    destRacks = [\n        protocol.load_labware(tubeRacks+tubeType, slot) for slot in range(\n            2, 2+numRacks)]\n\n    # Functions and Class creation\n    if not protocol.is_simulating:\n        class WellH(Well):\n            def __init__(self, well, height=0, min_height=5, comp_coeff=1.15,\n                         current_volume=0):\n                # Change one is that we deprecated well._impl\n                super().__init__(well.parent, well._core, APIVersion(2, 13))\n                self.well = well\n                self.height = height\n                self.min_height = min_height\n                self.comp_coeff = comp_coeff\n                self.radius = self.diameter / 2\n                self.current_volume = current_volume\n\n            def height_dec(self, vol):\n                dh = (vol / (math.pi * (self.radius ** 2))) * self.comp_coeff\n                if self.height - dh > self.min_height:\n                    self.height = self.height - dh\n                else:\n                    self.height = self.min_height\n                if self.current_volume - vol > 0:\n                    self.current_volume = self.current_volume - vol\n                else:\n                    self.current_volume = 0\n                return (self.well.bottom(self.height))\n\n            def height_inc(self, vol):\n                dh = (vol / (math.pi * (self.radius ** 2))) * self.comp_coeff\n                if self.height + dh < self.depth:\n                    self.height = self.height + dh\n                else:\n                    self.height = self.depth\n                self.current_volume += vol\n                return (self.well.bottom(self.height + 20))\n\n        def yield_groups(list, num):\n            \"\"\"\n            yield lists based on number of items\n            \"\"\"\n            for i in range(0, len(list), num):\n                yield list[i:i+num]\n\n        # get number of distributes pipette can handle\n        distribute_num = pip.max_volume // transferVol\n        if distribute_num < 1:\n            # if transfer volume is greater than pipette max volume,\n            # use Transfer mode\n            dispMode = 'Transfer'\n\n        # create source location with WellH\n        source = WellH(\n            srcLabware.wells()[0], min_height=3, current_volume=srcVol*1000)\n\n        # Protocol: Tube filling\n        pip.pick_up_tip()\n        all_wells = [well for tuberack in destRacks for well in tuberack.wells()]\n        if dispMode == 'Transfer':\n            for dest in all_wells:\n                pip.transfer(\n                    transferVol, source.height_dec(transferVol), dest,\n                    touch_tip=touchTip, new_tip='never')\n        else:\n            well_groups = list(yield_groups(all_wells, int(distribute_num)))\n            for wells in well_groups:\n                pip.distribute(\n                    transferVol, source.height_dec(transferVol), wells,\n                    blow_out=source, touch_tip=touchTip, new_tip='never')\n        pip.drop_tip()\n",
+    "content": "import math\nfrom opentrons.protocol_api.labware import Well\n\nfrom opentrons import APIVersion\n\nmetadata = {\n    'protocolName': 'Tube Filling',\n    'author': 'Opentrons <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(protocol):\n    [transferVol, tubeRacks, tubeType, numRacks, srcType, srcVol,\n     pipType, pipMnt, startTip, dispMode, touchTip] = get_values(  # noqa: F821\n     'transferVol', 'tubeRacks', 'tubeType', 'numRacks', 'srcType', 'srcVol',\n     'pipType', 'pipMnt', 'startTip', 'dispMode', 'touchTip')\n\n    # Load labware and pipettes\n\n    tipNum = pipType.split('_')[0][1:]\n    tipNum = '300' if tipNum == '50' else tipNum\n    tipType = f'opentrons_96_tiprack_{tipNum}ul'\n    tips = [protocol.load_labware(tipType, '11')]\n    pip = protocol.load_instrument(pipType, pipMnt, tip_racks=tips)\n\n    firstTip = startTip[0].upper()+str(int(startTip[1:]))\n    pip.starting_tip = tips[0][firstTip]\n\n    srcLabware = protocol.load_labware(srcType, '1')\n\n    destRacks = [\n        protocol.load_labware(tubeRacks+tubeType, slot) for slot in range(\n            2, 2+numRacks)]\n\n    # Functions and Class creation\n    if not protocol.is_simulating:\n        class WellH(Well):\n            def __init__(self, well, height=0, min_height=5, comp_coeff=1.15,\n                         current_volume=0):\n                # Change one is that we deprecated well._impl\n                super().__init__(well.parent, well._core, APIVersion(2, 13))\n                self.well = well\n                self.height = height\n                self.min_height = min_height\n                self.comp_coeff = comp_coeff\n                self.radius = self.diameter / 2\n                self.current_volume = current_volume\n\n            def height_dec(self, vol):\n                dh = (vol / (math.pi * (self.radius ** 2))) * self.comp_coeff\n                if self.height - dh > self.min_height:\n                    self.height = self.height - dh\n                else:\n                    self.height = self.min_height\n                if self.current_volume - vol > 0:\n                    self.current_volume = self.current_volume - vol\n                else:\n                    self.current_volume = 0\n                return (self.well.bottom(self.height))\n\n            def height_inc(self, vol):\n                dh = (vol / (math.pi * (self.radius ** 2))) * self.comp_coeff\n                if self.height + dh < self.depth:\n                    self.height = self.height + dh\n                else:\n                    self.height = self.depth\n                self.current_volume += vol\n                return (self.well.bottom(self.height + 20))\n\n        def yield_groups(list, num):\n            \"\"\"\n            yield lists based on number of items\n            \"\"\"\n            for i in range(0, len(list), num):\n                yield list[i:i+num]\n\n        # get number of distributes pipette can handle\n        distribute_num = pip.max_volume // transferVol\n        if distribute_num < 1:\n            # if transfer volume is greater than pipette max volume,\n            # use Transfer mode\n            dispMode = 'Transfer'\n\n        # create source location with WellH\n        source = WellH(\n            srcLabware.wells()[0], min_height=3, current_volume=srcVol*1000)\n\n        # Protocol: Tube filling\n        pip.pick_up_tip()\n        all_wells = [well for tuberack in destRacks\n                     for well in tuberack.wells()]\n        if dispMode == 'Transfer':\n            for dest in all_wells:\n                pip.transfer(\n                    transferVol, source.height_dec(transferVol), dest,\n                    touch_tip=touchTip, new_tip='never')\n        else:\n            well_groups = list(yield_groups(all_wells, int(distribute_num)))\n            for wells in well_groups:\n                pip.distribute(\n                    transferVol, source.height_dec(transferVol), wells,\n                    blow_out=source, touch_tip=touchTip, new_tip='never')\n        pip.drop_tip()\n",
     "custom_labware_defs": [
         {
             "brand": {

protocols/4a0be6/4a0be6.ot2.apiv2.py

@@ -89,7 +89,8 @@ def yield_groups(list, num):
 
         # Protocol: Tube filling
         pip.pick_up_tip()
-        all_wells = [well for tuberack in destRacks for well in tuberack.wells()]
+        all_wells = [well for tuberack in destRacks
+                     for well in tuberack.wells()]
         if dispMode == 'Transfer':
             for dest in all_wells:
                 pip.transfer(