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Add mix and blowout for sample transfer
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parrishpayne12 committed Aug 10, 2023
1 parent 4d971a9 commit dc725f1
Showing 1 changed file with 5 additions and 0 deletions.
5 changes: 5 additions & 0 deletions protocols/08207f/08207f.ot2.apiv2.py
Original file line number Diff line number Diff line change
Expand Up @@ -119,15 +119,20 @@ def slow_withdraw(pip, well, delay_seconds=2.0):
dest_well = dest_plate.wells_by_name()[well]

volume = float(row[6])
mix_reps = 2

if volume > 20:
p300.pick_up_tip()
p300.aspirate(volume, source_well)
p300.dispense(volume, dest_well)
p300.mix(mix_reps, 20)
p300.blowout()
p300.drop_tip()

else:
p20.pick_up_tip()
p20.aspirate(volume, source_well)
p20.dispense(volume, dest_well)
p20.mix(mix_reps, 10)
p20.blowout()
p20.drop_tip()

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