-
Notifications
You must be signed in to change notification settings - Fork 54
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
- Loading branch information
“ramifarawi”
committed
Nov 13, 2023
1 parent
99cfd79
commit d426b90
Showing
2 changed files
with
3 additions
and
3 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -1,5 +1,5 @@ | ||
| { | ||
| "content": "metadata = {\n 'protocolName': 'Cerillo Plate Reader Protocol',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n [csv_stock, csv_buff, dilute_stock, sol_vol,\n p300_mount, m300_mount] = get_values( # noqa: F821\n \"csv_stock\", \"csv_buff\", \"dilute_stock\", \"sol_vol\",\n \"p300_mount\", \"m300_mount\")\n\n# csv_stock = \"\"\"\n# x,1,2,3,4,5,6,7,8,9,10,11,12\n# A,x,20,20,20,20,20,20,20,20,20,20,20\n# B,X,40,40,40,40,40,40,40,40,40,40,40\n# C,x,29,29,29,29,29,29,29,29,29,29,29\n# D,40,40,40,40,40,40,40,40,40,40,40,40\n# E,29,29,29,29,29,29,29,29,29,29,29,29\n# F,40,40,40,40,40,40,40,40,40,40,40,40\n# G,29,29,29,29,29,29,29,29,29,29,29,29\n# H,40,40,40,40,40,40,40,40,40,40,40,40\n# \"\"\"\n#\n# csv_buff = \"\"\"\n# x,1,2,3,4,5,6,7,8,9,10,11,12\n# A,20,40,34,20,22,44,89,90,92,29,84,29\n# B,74,29,49,72,49,32,89,29,88,44,22,40\n# C,20,40,34,20,22,44,89,90,92,29,84,29\n# D,74,29,49,72,49,32,89,29,88,44,22,40\n# E,20,40,34,20,22,44,89,90,92,29,84,29\n# F,74,29,49,72,49,32,89,29,88,44,22,40\n# G,20,40,34,20,22,44,89,90,92,29,84,29\n# H,74,29,49,72,49,32,89,29,88,44,22,40\n# \"\"\"\n\n # labware\n reservoir = ctx.load_labware('nest_12_reservoir_15ml', 5)\n plate_reader = ctx.load_labware('cerillo_stratus_armadillo_flatbottom_200ul', 1)\n deepwell = ctx.load_labware('nest_96_wellplate_2ml_deep', 3)\n tips = [ctx.load_labware('opentrons_96_filtertiprack_200ul', slot)\n for slot in [7, 8, 9]]\n\n # pipettes\n m300 = ctx.load_instrument('p300_multi_gen2', m300_mount, tip_racks=tips)\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount, tip_racks=tips)\n\n csv_lines_stock = [[val.strip() for val in line.split(',')][1:]\n for line in csv_stock.splitlines()\n if line.split(',')[0].strip()][1:]\n\n csv_lines_buff = [[val.strip() for val in line.split(',')][1:]\n for line in csv_buff.splitlines()\n if line.split(',')[0].strip()][1:]\n\n # mapping\n stock_solution = reservoir.wells()[0]\n buffer = reservoir.wells()[1]\n cells = reservoir.wells()[-1]\n\n if dilute_stock:\n\n # protocol\n ctx.comment('\\n---------------ADDING BUFFER TO PLATE--------------\\n\\n')\n p300.pick_up_tip()\n for line, row in zip(csv_lines_buff, deepwell.rows()):\n for well_vol, well_name in zip(line, row):\n if well_vol.lower() == 'x':\n continue\n well_vol = int(well_vol)\n dest_well = well_name\n p300.aspirate(well_vol, buffer)\n p300.dispense(well_vol, dest_well)\n p300.drop_tip()\n\n ctx.comment('\\n---------------ADDING STOCK TO PLATE--------------\\n\\n')\n p300.pick_up_tip()\n for line, row in zip(csv_lines_stock, deepwell.rows()):\n for well_vol, well_name in zip(line, row):\n if well_vol.lower() == 'x':\n continue\n well_vol = int(well_vol)\n dest_well = well_name\n p300.aspirate(well_vol, stock_solution)\n p300.dispense(well_vol, dest_well.top())\n p300.drop_tip()\n\n ctx.comment('\\n-------------Mixing solution and stock------------\\n\\n')\n for s, d in zip(deepwell.rows()[0], plate_reader.rows()[0]):\n m300.pick_up_tip()\n m300.mix(5, 50, s)\n m300.aspirate(sol_vol, s)\n m300.dispense(sol_vol, d)\n m300.drop_tip()\n\n else:\n ctx.comment('\\n-----------Transferring solution to reader-------\\n\\n')\n for s, d in zip(deepwell.rows()[0], plate_reader.rows()[0]):\n m300.pick_up_tip()\n m300.aspirate(sol_vol, s)\n m300.dispense(sol_vol, d)\n m300.drop_tip()\n\n ctx.comment('\\n-------------Transferring cells to reader-------------\\n\\n')\n m300.pick_up_tip()\n m300.mix(20, 200, cells)\n for col in plate_reader.rows()[0]:\n m300.aspirate(180, cells)\n m300.dispense(180, col)\n m300.mix(3, 150, col)\n m300.drop_tip()\n", | ||
| "content": "metadata = {\n 'protocolName': 'Cerillo Plate Reader Protocol',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n [csv_stock, csv_buff, dilute_stock, sol_vol,\n p300_mount, m300_mount] = get_values( # noqa: F821\n \"csv_stock\", \"csv_buff\", \"dilute_stock\", \"sol_vol\",\n \"p300_mount\", \"m300_mount\")\n\n# csv_stock = \"\"\"\n# x,1,2,3,4,5,6,7,8,9,10,11,12\n# A,x,20,20,20,20,20,20,20,20,20,20,20\n# B,X,40,40,40,40,40,40,40,40,40,40,40\n# C,x,29,29,29,29,29,29,29,29,29,29,29\n# D,40,40,40,40,40,40,40,40,40,40,40,40\n# E,29,29,29,29,29,29,29,29,29,29,29,29\n# F,40,40,40,40,40,40,40,40,40,40,40,40\n# G,29,29,29,29,29,29,29,29,29,29,29,29\n# H,40,40,40,40,40,40,40,40,40,40,40,40\n# \"\"\"\n#\n# csv_buff = \"\"\"\n# x,1,2,3,4,5,6,7,8,9,10,11,12\n# A,20,40,34,20,22,44,89,90,92,29,84,29\n# B,74,29,49,72,49,32,89,29,88,44,22,40\n# C,20,40,34,20,22,44,89,90,92,29,84,29\n# D,74,29,49,72,49,32,89,29,88,44,22,40\n# E,20,40,34,20,22,44,89,90,92,29,84,29\n# F,74,29,49,72,49,32,89,29,88,44,22,40\n# G,20,40,34,20,22,44,89,90,92,29,84,29\n# H,74,29,49,72,49,32,89,29,88,44,22,40\n# \"\"\"\n\n # labware\n reservoir = ctx.load_labware('nest_12_reservoir_15ml', 5)\n plate_reader = ctx.load_labware('cerillo_stratus_armadillo_flatbottom_200ul', 1) # noqa: E501\n deepwell = ctx.load_labware('nest_96_wellplate_2ml_deep', 3)\n tips = [ctx.load_labware('opentrons_96_filtertiprack_200ul', slot)\n for slot in [7, 8, 9]]\n\n # pipettes\n m300 = ctx.load_instrument('p300_multi_gen2', m300_mount, tip_racks=tips)\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount, tip_racks=tips)\n\n csv_lines_stock = [[val.strip() for val in line.split(',')][1:]\n for line in csv_stock.splitlines()\n if line.split(',')[0].strip()][1:]\n\n csv_lines_buff = [[val.strip() for val in line.split(',')][1:]\n for line in csv_buff.splitlines()\n if line.split(',')[0].strip()][1:]\n\n # mapping\n stock_solution = reservoir.wells()[0]\n buffer = reservoir.wells()[1]\n cells = reservoir.wells()[-1]\n\n if dilute_stock:\n\n # protocol\n ctx.comment('\\n---------------ADDING BUFFER TO PLATE-------------\\n\\n')\n p300.pick_up_tip()\n for line, row in zip(csv_lines_buff, deepwell.rows()):\n for well_vol, well_name in zip(line, row):\n if well_vol.lower() == 'x':\n continue\n well_vol = int(well_vol)\n dest_well = well_name\n p300.aspirate(well_vol, buffer)\n p300.dispense(well_vol, dest_well)\n p300.drop_tip()\n\n ctx.comment('\\n---------------ADDING STOCK TO PLATE--------------\\n\\n')\n p300.pick_up_tip()\n for line, row in zip(csv_lines_stock, deepwell.rows()):\n for well_vol, well_name in zip(line, row):\n if well_vol.lower() == 'x':\n continue\n well_vol = int(well_vol)\n dest_well = well_name\n p300.aspirate(well_vol, stock_solution)\n p300.dispense(well_vol, dest_well.top())\n p300.drop_tip()\n\n ctx.comment('\\n-------------Mixing solution and stock------------\\n\\n')\n for s, d in zip(deepwell.rows()[0], plate_reader.rows()[0]):\n m300.pick_up_tip()\n m300.mix(5, 50, s)\n m300.aspirate(sol_vol, s)\n m300.dispense(sol_vol, d)\n m300.drop_tip()\n\n else:\n ctx.comment('\\n-----------Transferring solution to reader-------\\n\\n')\n for s, d in zip(deepwell.rows()[0], plate_reader.rows()[0]):\n m300.pick_up_tip()\n m300.aspirate(sol_vol, s)\n m300.dispense(sol_vol, d)\n m300.drop_tip()\n\n ctx.comment('\\n-------------Transferring cells to reader-------------\\n\\n')\n m300.pick_up_tip()\n m300.mix(20, 200, cells)\n for col in plate_reader.rows()[0]:\n m300.aspirate(180, cells)\n m300.dispense(180, col)\n m300.mix(3, 150, col)\n m300.drop_tip()\n", | ||
| "custom_labware_defs": [ | ||
| { | ||
| "brand": { | ||
|
|
||
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters