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“ramifarawi” committed Mar 1, 2024
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4 changes: 2 additions & 2 deletions protoBuilds/1033g1/README.json
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"deck-setup": "\n\nDeck set up at Day 4 when the Viability and Cytotoxicity Assay is carried out\ndeck\n\n\nDeck Setup after 72 hours when the Cell Toxicity and Viability assays are to be carried out\nSlot 1 Heater Shaker Module- Plate at 23\u00b0C(deactivated) and after reagent addition, the orbital shaking is set to 500 rpm for 2 min.\nSlot 4 Opentrons 96 Filter Tip rack 200\u03bcl\nSlot 10 Opentrons 96 Filter Tip rack 20\u03bcl\nSlot 11 Opentrons 10 Tube Rack with Falcon 4 X 50 mL, 6X15mL Conical-Rack for holding Cell Titer Glo 2.0 Reagent and CellTox Green reagent.\n\n",
"description": "This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96-well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.",
"description": "This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96 well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.",
"internal": "1033g1",
"labware": "\nCorning 96 Well Plate 360 \u00b5L Flat #3650\nOpentrons 96 Tip Rack 300 \u00b5L\nOpentrons 96 Filter Tip Rack 20 \u00b5L\nOpentrons 10 Tube Rack with Falcon 4x50 mL, 6x15 mL Conical\n",
"markdown": {
"author": "Vasudha Nair\n\n\n",
"categories": "* Cell and Tissue Culture\n\t* Cell and Tissue Culture\n\n\n",
"deck-setup": "* Deck set up at Day 4 when the Viability and Cytotoxicity Assay is carried out\n[deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/1033g1/Screen+Shot+2024-03-01+at+9.13.29+AM.png)\n\n* Deck Setup after 72 hours when the Cell Toxicity and Viability assays are to be carried out\nSlot 1 Heater Shaker Module- Plate at 23\u00b0C(deactivated) and after reagent addition, the orbital shaking is set to 500 rpm for 2 min.\nSlot 4 Opentrons 96 Filter Tip rack 200\u03bcl\nSlot 10 Opentrons 96 Filter Tip rack 20\u03bcl\nSlot 11 Opentrons 10 Tube Rack with Falcon 4 X 50 mL, 6X15mL Conical-Rack for holding Cell Titer Glo 2.0 Reagent and CellTox Green reagent.\n\n\n\n",
"description": "This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96-well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.\n\n\n",
"description": "This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96 well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.\n\n\n",
"internal": "1033g1\n",
"labware": "* [Corning 96 Well Plate 360 \u00b5L Flat #3650](https://ecatalog.corning.com/life-sciences/b2c/US/en/Microplates/Assay-Microplates/96-Well-Microplates/Corning%C2%AE-96-well-Solid-Black-and-White-Polystyrene-Microplates/p/corning96WellSolidBlackAndWhitePolystyreneMicroplates)\n* [Opentrons 96 Tip Rack 300 \u00b5L](https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-300ul-tips)\n* Opentrons 96 Filter Tip Rack 20 \u00b5L\n* [Opentrons 10 Tube Rack with Falcon 4x50 mL, 6x15 mL Conical](https://shop.opentrons.com/collections/opentrons-tips/products/tube-rack-set-1)\n\n\n",
"notes": "If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/).\n\n\n",
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2 changes: 1 addition & 1 deletion protocols/1033g1/README.md
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## Description
This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96-well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.
This protocol can be used to measure the viability and cytotoxicity of two different cell types (suspension and adherent) that have been treated with respective drugs, using Cell Viability assay kit (Cell Titer Glo 2.0) and Cytotoxicity assay kit (CellTox Green) from Promega on the OT-2. This protocol is designed for the 96 well plate format and both assays can be processed in the same plate. In the case of K562 cells, the protocol is broken down into 2 main parts: a) Seeding/ Plating of K562 cells and bortezomib additions to the cells b) After 72 hours, completion of both the assays in the same plate i.e. sequential multiplexing of CellTox Green Cytotoxicity Assay and CellTiter Glo 2.0 Assay.


### Labware
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