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{ | ||
"author": "Opentrons\nOpentrons has launched a new Protocol Library. You should use the new page for this protocol. This page won\u2019t be available after March 31st, 2024.", | ||
"author": "Opentrons", | ||
"categories": { | ||
"Sample Prep": [ | ||
"Cherrypicking" | ||
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@@ -8,7 +8,7 @@ | |
"description": "\nCherrypicking, or hit-picking, is a key component of many workflows from high-throughput screening to microbial transfections. With this protocol, you can easily select specific wells in any labware without worrying about missing or selecting the wrong well. Just upload your properly formatted CSV file (keep scrolling for an example), customize your parameters, and download your ready-to-run protocol.\n\n\nTo purchase tips, reagents, or pipettes, please visit our online store or contact our sales team at [email protected]\n\nOpentrons OT-2\nOpentrons OT-2 Run App (Version 3.15.0 or later)\nOpentrons Single-Channel Pipette and corresponding Tips\nMicroplates (96-well or 384-well)\n\nFor more detailed information on compatible labware, please visit our Labware Library.\n\n\nLabware will be loaded automatically by specifying the labware loadname and labware slot in the .csv file. All available empty slots will be filled with the necessary tipracks, and the user will be prompted to refill the tipracks if all are emptied in the middle of the protocol.\nCSV Format\nYour cherrypicking transfers must be saved as a comma separated value (.csv) file type. Your CSV must contain values corresponding to volumes in microliters (\u03bcL). Note that the header line (first row of the .csv file) should also be included!\nHere's an example of how a short cherrypicking protocol should be properly formatted:\nNew Tip,Source Labware,Source Slot,Source Well,Source Aspiration Height Above Bottom (in mm),Dest Labware,Dest Slot,Dest Well,Dest Dispense Height Above Bottom (in mm),Volume (in ul)\nyes,agilent_1_reservoir_290ml,1,A1,1,nest_96_wellplate_100ul_pcr_full_skirt,4,A11,2,1\n,nest_12_reservoir_15ml,2,A1,1,corning_384_wellplate_112ul_flat,5,B11,2,3\nyes,nest_1_reservoir_195ml,3,A1,1,corning_384_wellplate_112ul_flat,5,A12,2,7\nIn the above example, 1\u03bcL will be transferred from 1mm above the bottom of well A1 in an Agilent 1-well 290ml reservoir (slot 1) to well A11 in the destination NEST 96-well plate 100\u00b5l (slot 4) with a new tip. After this, 3\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 12-well 15ml reservoir (slot 2) to well A5 in the destination NEST 96-well plate 100\u00b5l (slot 5) with the same tip. Last, 7\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 1-well 195ml reservoir (slot 3) to well H12 in the destination NEST 96-well plate 100\u00b5l (slot 5) with a new tip.\nIf you\u2019d like to follow our template, you can make a copy of this spreadsheet, fill out your values, and export as CSV for use with this protocol.\nUsing the customizations fields, below set up your protocol.\n Transfer .csv File: Upload the .csv file containing your well locations, volumes, and source plate (optional).\n Pipette Model: Select which pipette you will use for this protocol.\n Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n Tip Type: Specify whether you want to use filter tips.\n* Tip Usage Strategy: Specify whether you'd like to use a new tip for each transfer, or keep the same tip throughout the protocol.", | ||
"internal": "274d2a", | ||
"markdown": { | ||
"author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/274d2a). This page won\u2019t be available after March 31st, 2024.\n\n", | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n", | ||
"categories": "* Sample Prep\n\t* Cherrypicking\n\n", | ||
"description": "![Cherrypicking Example](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/cherrypicking/cherrypicking_example.png)\n\nCherrypicking, or hit-picking, is a key component of many workflows from high-throughput screening to microbial transfections. With this protocol, you can easily select specific wells in any labware without worrying about missing or selecting the wrong well. Just upload your properly formatted CSV file (keep scrolling for an example), customize your parameters, and download your ready-to-run protocol.\n\n---\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/materials.png)\n\nTo purchase tips, reagents, or pipettes, please visit our [online store](https://shop.opentrons.com/) or contact our sales team at [[email protected]](mailto:[email protected])\n\n* [Opentrons OT-2](https://shop.opentrons.com/collections/ot-2-robot/products/ot-2)\n* [Opentrons OT-2 Run App (Version 3.15.0 or later)](https://opentrons.com/ot-app/)\n* [Opentrons Single-Channel Pipette](https://shop.opentrons.com/collections/ot-2-pipettes) and corresponding [Tips](https://shop.opentrons.com/collections/opentrons-tips)\n* [Microplates (96-well or 384-well)](https://labware.opentrons.com/?category=wellPlate)\n\nFor more detailed information on compatible labware, please visit our [Labware Library](https://labware.opentrons.com/).\n\n\n\n---\n![Setup](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/Setup.png)\n\nLabware will be loaded automatically by specifying the labware loadname and labware slot in the .csv file. All available empty slots will be filled with the necessary [tipracks](https://shop.opentrons.com/collections/opentrons-tips), and the user will be prompted to refill the tipracks if all are emptied in the middle of the protocol.\n\n**CSV Format**\n\nYour cherrypicking transfers must be saved as a comma separated value (.csv) file type. Your CSV must contain values corresponding to volumes in microliters (\u03bcL). Note that the header line (first row of the .csv file) should also be included!\n\nHere's an example of how a short cherrypicking protocol should be properly formatted:\n\n```\nNew Tip,Source Labware,Source Slot,Source Well,Source Aspiration Height Above Bottom (in mm),Dest Labware,Dest Slot,Dest Well,Dest Dispense Height Above Bottom (in mm),Volume (in ul)\nyes,agilent_1_reservoir_290ml,1,A1,1,nest_96_wellplate_100ul_pcr_full_skirt,4,A11,2,1\n,nest_12_reservoir_15ml,2,A1,1,corning_384_wellplate_112ul_flat,5,B11,2,3\nyes,nest_1_reservoir_195ml,3,A1,1,corning_384_wellplate_112ul_flat,5,A12,2,7\n```\n\nIn the above example, 1\u03bcL will be transferred from 1mm above the bottom of well A1 in an Agilent 1-well 290ml reservoir (slot 1) to well A11 in the destination NEST 96-well plate 100\u00b5l (slot 4) with a new tip. After this, 3\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 12-well 15ml reservoir (slot 2) to well A5 in the destination NEST 96-well plate 100\u00b5l (slot 5) with the same tip. Last, 7\u03bcL will be transferred from 1mm above the bottom of well A1 in a NEST 1-well 195ml reservoir (slot 3) to well H12 in the destination NEST 96-well plate 100\u00b5l (slot 5) with a new tip.\n\nIf you\u2019d like to follow our template, you can make a copy of [this spreadsheet](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/274d2a/ex.csv), fill out your values, and export as CSV for use with this protocol.\n\nUsing the customizations fields, below set up your protocol.\n* Transfer .csv File: Upload the .csv file containing your well locations, volumes, and source plate (optional).\n* Pipette Model: Select which pipette you will use for this protocol.\n* Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n* Tip Type: Specify whether you want to use filter tips.\n* Tip Usage Strategy: Specify whether you'd like to use a new tip for each transfer, or keep the same tip throughout the protocol.\n\n\n", | ||
"internal": "274d2a\n", | ||
|
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Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -1,5 +1,5 @@ | ||
{ | ||
"author": "Opentrons\nOpentrons has launched a new Protocol Library. You should use the new page for this protocol. This page won\u2019t be available after March 31st, 2024.", | ||
"author": "Opentrons", | ||
"categories": { | ||
"Getting Started": [ | ||
"Opentrons Logo" | ||
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@@ -8,7 +8,7 @@ | |
"description": "This is a demo protocol that will help you to get more familiar with your new OT-2! All you need is some food dye, a 96-well plate, and a 12-row trough or tube rack with 1.5mL or 2mL tubes. Your robot will pipette the Opentrons logo into your plate and you'll be ready to go!\n\n\nTo purchase tips, reagents, or pipettes, please visit our online store or contact our sales team at [email protected]\n\nOpentrons OT-2\nOpentrons OT-2 Run App (Version 3.15.0 or later)\nOpentrons Single-Channel Pipette and corresponding Tips\n96-Well Microplate\n12-Row Trough or Tube Rack with 1.5mL/2mL Tubes\nWater and Food Dye (Two Colors)\n\nFor more detailed information on compatible labware, please visit our Labware Library.\n\n\nFor this demo protocol, you need a clean, empty 96-well plate (where the Opentrons logo will be created) and either a 12-row trough or 1.5mL/2mL tubes in a tube rack to store the water and food dye solution.\nIf using a 12-row trough, the two food dye solutions should be stored in column 1 and column 2. If using the tube rack, dye 1 should be stored in 'A1' & 'B1' and dye 2 should be stored in 'C1' & 'D1'.\nUsing the customization fields below, set up your protocol.\n Pipette Model: Select which pipette you will use for this protocol.\n Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n Destination Plate Type: Select which (destination) plate you will use for this protocol.\n Dye Labware Type: Select which (source) labware you will use for this protocol.", | ||
"internal": "Demo Protocol 1", | ||
"markdown": { | ||
"author": "[Opentrons](https://opentrons.com/)\n\n# Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](https://library.opentrons.com/p/opentrons_logo). This page won\u2019t be available after March 31st, 2024.\n\n", | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n", | ||
"categories": "* Getting Started\n\t* Opentrons Logo\n\n", | ||
"description": "This is a demo protocol that will help you to get more familiar with your new OT-2! All you need is some food dye, a 96-well plate, and a 12-row trough or tube rack with 1.5mL or 2mL tubes. Your robot will pipette the Opentrons logo into your plate and you'll be ready to go!\n\n---\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/materials.png)\n\nTo purchase tips, reagents, or pipettes, please visit our [online store](https://shop.opentrons.com/) or contact our sales team at [[email protected]](mailto:[email protected])\n\n* [Opentrons OT-2](https://shop.opentrons.com/collections/ot-2-robot/products/ot-2)\n* [Opentrons OT-2 Run App (Version 3.15.0 or later)](https://opentrons.com/ot-app/)\n* [Opentrons Single-Channel Pipette](https://shop.opentrons.com/collections/ot-2-pipettes) and corresponding [Tips](https://shop.opentrons.com/collections/opentrons-tips)\n* [96-Well Microplate](https://labware.opentrons.com/?category=wellPlate)\n* [12-Row Trough](https://labware.opentrons.com/?category=reservoir) or [Tube Rack with 1.5mL/2mL Tubes](https://labware.opentrons.com/?category=tubeRack)\n* Water and Food Dye (Two Colors)\n\nFor more detailed information on compatible labware, please visit our [Labware Library](https://labware.opentrons.com/).\n\n\n---\n![Setup](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/Setup.png)\n\nFor this demo protocol, you need a clean, empty 96-well plate (where the Opentrons logo will be created) and either a 12-row trough or 1.5mL/2mL tubes in a tube rack to store the water and food dye solution.\n\nIf using a 12-row trough, the two food dye solutions should be stored in column 1 and column 2. If using the tube rack, dye 1 should be stored in 'A1' & 'B1' and dye 2 should be stored in 'C1' & 'D1'.\n\nUsing the customization fields below, set up your protocol.\n* Pipette Model: Select which pipette you will use for this protocol.\n* Pipette Mount: Specify which mount your single-channel pipette is on (left or right)\n* Destination Plate Type: Select which (destination) plate you will use for this protocol.\n* Dye Labware Type: Select which (source) labware you will use for this protocol.\n\n\n", | ||
"internal": "Demo Protocol 1\n", | ||
|
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