fxi

protoBuilds/1464-3/ELISA_protocol_part3.ot2.apiv2.py.json

@@ -1,5 +1,5 @@
 {
-    "content": "metadata = {\n    'protocolName': 'ELISA',\n    'author': 'Alise <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n    [number_of_columns] = get_values(  # noqa: F821\n     'number_of_columns')\n\n    if number_of_columns > 12:\n        raise Exception('The number of columns cannot exceed 12.')\n    if number_of_columns % 2 == 1:\n        raise Exception('The number of columns should be even.')\n\n    # labware setup\n    trough = ctx.load_labware('nest_12_reservoir_15ml', '8')\n    plate = ctx.load_labware('corning_96_wellplate_360ul_flat', '9')\n\n    tiprack_m300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n                    for slot in ['3', '7']]\n\n    # instrument setup\n    m300 = ctx.load_instrument(\n        'p300_multi_gen2',\n        mount='left',\n        tip_racks=tiprack_m300)\n\n    # reagent setup\n    TMB_substrate = trough.wells_by_name()['A6']\n    stop_solution = trough.wells_by_name()['A12']\n\n    \"\"\"\n    Adding TMB substrate\n    \"\"\"\n    m300.pick_up_tip()\n    m300.distribute(\n        100,\n        TMB_substrate.bottom(),\n        [col[0].top() for col in plate.columns()[:number_of_columns]],\n        blow_out=TMB_substrate,\n        new_tip='never')\n    m300.aspirate(20, plate.columns()[number_of_columns-1][0].top())\n    m300.drop_tip()\n\n    ctx.delay(minutes=30)\n\n    \"\"\"\n    Adding Stop Solution\n    \"\"\"\n    for col in plate.columns()[:number_of_columns]:\n        m300.pick_up_tip()\n        m300.transfer(100, stop_solution.bottom(), col, new_tip='never')\n        m300.drop_tip()\n",
+    "content": "metadata = {\n    'protocolName': 'Substrate and Stop Solution Addition',\n    'author': 'Alise <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n    [number_of_columns] = get_values(  # noqa: F821\n     'number_of_columns')\n\n    if number_of_columns > 12:\n        raise Exception('The number of columns cannot exceed 12.')\n    if number_of_columns % 2 == 1:\n        raise Exception('The number of columns should be even.')\n\n    # labware setup\n    trough = ctx.load_labware('nest_12_reservoir_15ml', '8')\n    plate = ctx.load_labware('corning_96_wellplate_360ul_flat', '9')\n\n    tiprack_m300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n                    for slot in ['3', '7']]\n\n    # instrument setup\n    m300 = ctx.load_instrument(\n        'p300_multi_gen2',\n        mount='left',\n        tip_racks=tiprack_m300)\n\n    # reagent setup\n    TMB_substrate = trough.wells_by_name()['A6'].bottom(z=0)\n    stop_solution = trough.wells_by_name()['A12'].bottom(z=0)\n\n    \"\"\"\n    Adding TMB substrate\n    \"\"\"\n    m300.pick_up_tip()\n    m300.distribute(\n        100,\n        TMB_substrate,\n        [col[0].top() for col in plate.columns()[:number_of_columns]],\n        blow_out=False,\n        disposal_volume=0.05,\n\n        new_tip='never')\n    m300.aspirate(20, plate.columns()[number_of_columns-1][0].top())\n    m300.drop_tip()\n\n    ctx.delay(minutes=30)\n\n    \"\"\"\n    Adding Stop Solution\n    \"\"\"\n    for col in plate.columns()[:number_of_columns]:\n        m300.pick_up_tip()\n        m300.transfer(100, stop_solution, col, new_tip='never')\n        m300.drop_tip()\n",
     "custom_labware_defs": [],
     "fields": [
         {
@@ -50,7 +50,7 @@
     "metadata": {
         "apiLevel": "2.13",
         "author": "Alise <[email protected]>",
-        "protocolName": "ELISA",
+        "protocolName": "Substrate and Stop Solution Addition",
         "source": "Custom Protocol Request"
     },
     "modules": []