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fix
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“ramifarawi” committed Mar 6, 2024
1 parent 90f4e79 commit 4dad96a
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2 changes: 1 addition & 1 deletion protoBuilds/0f7910/0f7910.ot2.apiv2.py.json
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Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
{
"content": "from opentrons import protocol_api\n\n\nmetadata = {\n 'protocolName': 'Plate Filling with CSV Import',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.11'\n}\n\n\ndef run(ctx):\n\n [csv_samp,\n source_format,\n dest_format,\n starting_tip,\n p300_mount,\n p20_mount] = get_values( # noqa: F821\n \"csv_samp\",\n \"source_format\",\n \"dest_format\",\n \"starting_tip\",\n \"p300_mount\",\n \"p20_mount\")\n\n # mapping\n csv_lines = [[val.strip() for val in line.split(',')]\n for line in csv_samp.splitlines()\n if line.split(',')[0].strip()][1:]\n\n unique_list = []\n for row in csv_lines:\n source_plate_slot = int(row[1])\n if source_plate_slot not in unique_list:\n unique_list.append(source_plate_slot)\n\n starting_tip -= starting_tip\n\n # labware\n source_plates = [ctx.load_labware(\n 'corning_96_wellplate_360ul_flat'\n if source_format == \"96\"\n else \"corning_384_wellplate_112ul_flat\", slot)\n for slot in unique_list]\n source_plates = source_plates\n\n dest_plate = ctx.load_labware(\n 'corning_96_wellplate_360ul_flat'\n if dest_format == \"96\"\n else \"corning_384_wellplate_112ul_flat\", 2\n if dest_format == \"384\"\n else 1)\n transfer_vol = float(csv_lines[0][5])\n\n if transfer_vol > 20:\n tips300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n for slot in [10]]\n else:\n tips20 = [ctx.load_labware('opentrons_96_tiprack_20ul', slot)\n for slot in [11]]\n\n # pipettes\n if transfer_vol > 20:\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n tip_racks=tips300)\n\n else:\n p20 = ctx.load_instrument('p20_single_gen2', p20_mount,\n tip_racks=tips20)\n\n def pick_up(pip):\n try:\n pip.pick_up_tip()\n except protocol_api.labware.OutOfTipsError:\n ctx.pause(\"Replace empty tip racks.\")\n pip.reset_tipracks()\n pick_up(pip)\n\n # protocol\n\n pip = p20 if transfer_vol <= 20 else p300\n pip.flow_rate.aspirate = 1\n pip.flow_rate.dispense = 2\n\n if transfer_vol > 20:\n pip.starting_tip = tips300[0].wells()[starting_tip]\n else:\n pip.starting_tip = tips20[0].wells()[starting_tip]\n\n for row in csv_lines:\n source_plate_slot = int(row[1])\n source_well_name = row[2]\n dest_well_name = row[4]\n pip_transfer_vol = float(row[5])\n\n source = ctx.loaded_labwares[source_plate_slot].wells_by_name()[source_well_name] # noqa: E501\n dest = dest_plate.wells_by_name()[dest_well_name]\n\n pick_up(pip)\n pip.transfer(pip_transfer_vol, source.bottom(z=0.2), dest,\n new_tip='never',\n blow_out=True, blowout_location='destination well')\n pip.drop_tip()\n ctx.comment('\\n\\n')\n",
"content": "from opentrons import protocol_api\n\n\nmetadata = {\n 'protocolName': 'Plate Filling with CSV Import',\n 'author': 'Rami Farawi <[email protected]>',\n 'source': 'Custom Protocol Request',\n 'apiLevel': '2.11'\n}\n\n\ndef run(ctx):\n\n [csv_samp,\n source_format,\n dest_format,\n starting_tip,\n p300_mount,\n p20_mount] = get_values( # noqa: F821\n \"csv_samp\",\n \"source_format\",\n \"dest_format\",\n \"starting_tip\",\n \"p300_mount\",\n \"p20_mount\")\n\n # mapping\n csv_lines = [[val.strip() for val in line.split(',')]\n for line in csv_samp.splitlines()\n if line.split(',')[0].strip()][1:]\n\n unique_list = []\n for row in csv_lines:\n source_plate_slot = int(row[1])\n if source_plate_slot not in unique_list:\n unique_list.append(source_plate_slot)\n\n starting_tip -= starting_tip\n\n # labware\n source_plates = [ctx.load_labware(\n 'corning_96_wellplate_360ul_flat'\n if source_format == \"96\"\n else \"corning_384_wellplate_112ul_flat\", slot)\n for slot in unique_list]\n source_plates = source_plates\n\n dest_plate = ctx.load_labware(\n 'corning_96_wellplate_360ul_flat'\n if dest_format == \"96\"\n else \"corning_384_wellplate_112ul_flat\", 2\n if dest_format == \"384\"\n else 1)\n transfer_vol = float(csv_lines[0][5])\n\n if transfer_vol > 20:\n tips300 = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n for slot in [10]]\n else:\n tips20 = [ctx.load_labware('opentrons_96_tiprack_20ul', slot)\n for slot in [11]]\n\n # pipettes\n if transfer_vol > 20:\n p300 = ctx.load_instrument('p300_single_gen2', p300_mount,\n tip_racks=tips300)\n\n else:\n p20 = ctx.load_instrument('p20_single_gen2', p20_mount,\n tip_racks=tips20)\n\n def pick_up(pip):\n try:\n pip.pick_up_tip()\n except protocol_api.labware.OutOfTipsError:\n ctx.pause(\"Replace empty tip racks.\")\n pip.reset_tipracks()\n pick_up(pip)\n\n # protocol\n\n pip = p20 if transfer_vol <= 20 else p300\n pip.flow_rate.aspirate = 1\n pip.flow_rate.dispense = 2\n\n if transfer_vol > 20:\n pip.starting_tip = tips300[0].wells()[starting_tip]\n else:\n pip.starting_tip = tips20[0].wells()[starting_tip]\n\n for row in csv_lines:\n source_plate_slot = int(row[1])\n source_well_name = row[2]\n dest_well_name = row[4]\n pip_transfer_vol = float(row[5])\n\n source = ctx.loaded_labwares[source_plate_slot].wells_by_name()[source_well_name] # noqa: E501\n dest = dest_plate.wells_by_name()[dest_well_name]\n\n pick_up(pip)\n pip.mix(1, pip_transfer_vol, source.bottom(z=0.2))\n pip.aspirate(pip_transfer_vol, source.bottom(z=0.2))\n pip.dispense(pip_transfer_vol, dest)\n pip.move_to(dest.top(z=-3))\n ctx.delay(seconds=1)\n pip.blow_out()\n pip.drop_tip()\n ctx.comment('\\n\\n')\n",
"custom_labware_defs": [],
"fields": [
{
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9 changes: 6 additions & 3 deletions protocols/0f7910/0f7910.ot2.apiv2.py
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Original file line number Diff line number Diff line change
Expand Up @@ -98,8 +98,11 @@ def pick_up(pip):
dest = dest_plate.wells_by_name()[dest_well_name]

pick_up(pip)
pip.transfer(pip_transfer_vol, source.bottom(z=0.2), dest,
new_tip='never',
blow_out=True, blowout_location='destination well')
pip.mix(1, pip_transfer_vol, source.bottom(z=0.2))
pip.aspirate(pip_transfer_vol, source.bottom(z=0.2))
pip.dispense(pip_transfer_vol, dest)
pip.move_to(dest.top(z=-3))
ctx.delay(seconds=1)
pip.blow_out()
pip.drop_tip()
ctx.comment('\n\n')

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