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edit ELUTION STEP
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parrishpayne12 committed Sep 11, 2023
1 parent 5b9f300 commit 35a9f37
Showing 1 changed file with 10 additions and 5 deletions.
15 changes: 10 additions & 5 deletions protocols/0ff0c3/0ff0c3.ot2.apiv2.py
Original file line number Diff line number Diff line change
Expand Up @@ -198,15 +198,20 @@ def slow_tip_withdrawal(current_pipette, well_location, to_center=False):
pick_up(m300)
m300.aspirate(50, elution_buff)
m300.dispense(50, col)
m300.mix(15, 50, col)

for i in range(20):
m300.aspirate(50, col.bottom(0.2), rate=2)
m300.dispense(50, col.bottom(2), rate=2)

#m300.mix(15, 50, col)
m300.drop_tip()

mag_mod.engage(height_from_base=4.0)
ctx.delay(minutes=5)
#May need to replace this with a for loop for the mixing
mag_mod.engage(height_from_base=3.5)
#ctx.delay(minutes=5)

for s, d in zip(samples, destination):
pick_up(m300)
m300.aspirate(50, s.bottom(0.4), rate=0.1)
m300.aspirate(50, s.bottom(0.1), rate=0.1)
m300.dispense(50, d)
m300.drop_tip()

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