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| { | ||
| "author": "Opentrons (verified)\nOpentrons has launched a new Protocol Library. You should use the new page for this protocol. This page won\u2019t be available after January 31st, 2024.", | ||
| "categories": { | ||
| "Nucleic Acid Extraction & Purification": [ | ||
| "RNA Extraction" | ||
| ] | ||
| }, | ||
| "deck-setup": "", | ||
| "description": "After lysing samples, your OT-2 can fully automate the entire Omega Bio-tek Mag-Bind\u00ae Blood & Tissue DNA HDQ 96 Kit. Buffer systems tailored specifically for each type of starting material are added to samples to undergo lysis. Samples are then mixed with HDQ Binding Buffer and Mag-Bind\u00ae Particles HDQ to bind magnetic beads to DNA. DNA is eluted in the Elution Buffer after rapid wash steps.\nResults of the Opentrons Science team's internal testing of this protocol on the OT-2 are shown below: \n\nExplanation of complex parameters below:\n Number of samples: Specify the number of samples this run (1-96 and divisible by 8, i.e. whole columns at a time).\n Deepwell type: Specify which well plate will be mounted on the magnetic module.\n Reservoir Type: Specify which reservoir will be employed.\n Starting Volume: Specify starting volume of sample (ul).\n Elution Volume: Specify elution volume (ul).\n Park Tips: Specify whether to park tips or drop tips.\n Mag Deck Generation: Specify whether GEN1 or GEN2 magnetic module will be used.\n P300 Multi Channel Pipette Mount: Specify whether the P300 multi channel pipette will be on the left or right mount.\n", | ||
| "internal": "sci-omegabiotek-extraction", | ||
| "labware": "\nNEST 96 Wellplate 2mL\nUSA Scientific 96 Wellplate 2.4mL\nNEST 12 Reservoir 15mL\nUSA Scientific 12 Reservoir 22mL\nOpentrons 96 tiprack 300ul\nOpentrons 96 Aluminum block Nest Wellplate 100ul\n", | ||
| "markdown": { | ||
| "author": "[Opentrons (verified)](https://opentrons.com/)\n\n***Opentrons has launched a new Protocol Library. You should use the [new page for this protocol](library.opentrons.com/p/sci-omegabiotek-extraction-fa). This page won\u2019t be available after January 31st, 2024.***\n\n", | ||
| "categories": "* Nucleic Acid Extraction & Purification\n\t* RNA Extraction\n\n", | ||
| "deck-setup": "\n\n\n", | ||
| "description": "After lysing samples, your OT-2 can fully automate the entire Omega Bio-tek Mag-Bind\u00ae Blood & Tissue DNA HDQ 96 Kit. Buffer systems tailored specifically for each type of starting material are added to samples to undergo lysis. Samples are then mixed with HDQ Binding Buffer and Mag-Bind\u00ae Particles HDQ to bind magnetic beads to DNA. DNA is eluted in the Elution Buffer after rapid wash steps.\n\nResults of the Opentrons Science team's internal testing of this protocol on the OT-2 are shown below: \n\n\n\nExplanation of complex parameters below:\n* `Number of samples`: Specify the number of samples this run (1-96 and divisible by 8, i.e. whole columns at a time).\n* `Deepwell type`: Specify which well plate will be mounted on the magnetic module.\n* `Reservoir Type`: Specify which reservoir will be employed.\n* `Starting Volume`: Specify starting volume of sample (ul).\n* `Elution Volume`: Specify elution volume (ul).\n* `Park Tips`: Specify whether to park tips or drop tips.\n* `Mag Deck Generation`: Specify whether GEN1 or GEN2 magnetic module will be used.\n* `P300 Multi Channel Pipette Mount`: Specify whether the P300 multi channel pipette will be on the left or right mount.\n\n\n---\n\n", | ||
| "internal": "sci-omegabiotek-extraction\n", | ||
| "labware": "* [NEST 96 Wellplate 2mL](https://shop.opentrons.com/collections/lab-plates/products/nest-0-2-ml-96-well-deep-well-plate-v-bottom)\n* [USA Scientific 96 Wellplate 2.4mL](https://labware.opentrons.com/?category=wellPlate)\n* [NEST 12 Reservoir 15mL](https://shop.opentrons.com/collections/reservoirs/products/nest-12-well-reservoir-15-ml)\n* [USA Scientific 12 Reservoir 22mL](https://labware.opentrons.com/?category=reservoir)\n* [Opentrons 96 tiprack 300ul](https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-300ul-tips)\n* [Opentrons 96 Aluminum block Nest Wellplate 100ul](https://labware.opentrons.com/opentrons_96_aluminumblock_nest_wellplate_100ul?category=aluminumBlock)\n\n", | ||
| "modules": "* [Magnetic Module (GEN2)](https://shop.opentrons.com/collections/hardware-modules/products/magdeck)\n\n\n", | ||
| "notes": "If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/).\n\n", | ||
| "pipettes": "* [P300 Multi Channel Pipette](https://shop.opentrons.com/collections/ot-2-robot/products/8-channel-electronic-pipette)\n\n", | ||
| "process": "1. Input your protocol parameters above.\n2. Download your protocol and unzip if needed.\n3. Upload your custom labware to the [OT App](https://opentrons.com/ot-app) by navigating to `More` > `Custom Labware` > `Add Labware`, and selecting your labware files (.json extensions) if needed.\n4. Upload your protocol file (.py extension) to the [OT App](https://opentrons.com/ot-app) in the `Protocol` tab.\n5. Set up your deck according to the deck map.\n6. Calibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our [support articles](https://support.opentrons.com/en/collections/1559720-guide-for-getting-started-with-the-ot-2).\n7. Hit 'Run'.\n\n", | ||
| "protocol-steps": "1. Binding buffer is mixed\n2. Binding buffer added to samples\n3. Binding buffer and sample mixed\n4. Samples will be mixed with parked tips\n5. Engage magnetic module\n6. Incubate 7 minutes\n7. Remove supernatant\n8. Wash with wash buffer 1 and mix\n9. Engage magnetic module\n10. Delay 7 minutes\n11. Remove supernatant\n12. Disengage magnet\n13. Repeat steps 8-11 with wash buffer 2 and 3\n14. Elution solution added to sample and mixed\n15. Delay 5 minutes for elution\n16. Elute added to aluminum block\n\n", | ||
| "reagent-setup": "\n* Reservoir 1: Slot 2\n\n\n\n* Reservoir 2: Slot 3\n\n\n\n---\n\n", | ||
| "reagents": "* [Omega Bio-tek](https://www.omegabiotek.com/product/mag-bind-hdq-blood-dna-96-kit/?cn-reloaded=1)\n\n---\n\n", | ||
| "title": "Mag-Bind\u00ae Blood & Tissue DNA HDQ 96 Kit (Fully Automated)" | ||
| }, | ||
| "modules": [ | ||
| "Magnetic Module (GEN2)" | ||
| ], | ||
| "notes": "If you have any questions about this protocol, please contact the Protocol Development Team by filling out the Troubleshooting Survey.", | ||
| "pipettes": "\nP300 Multi Channel Pipette\n", | ||
| "process": "\nInput your protocol parameters above.\nDownload your protocol and unzip if needed.\nUpload your custom labware to the OT App by navigating to More > Custom Labware > Add Labware, and selecting your labware files (.json extensions) if needed.\nUpload your protocol file (.py extension) to the OT App in the Protocol tab.\nSet up your deck according to the deck map.\nCalibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our support articles.\nHit 'Run'.\n", | ||
| "protocol-steps": "\nBinding buffer is mixed\nBinding buffer added to samples\nBinding buffer and sample mixed\nSamples will be mixed with parked tips\nEngage magnetic module\nIncubate 7 minutes\nRemove supernatant\nWash with wash buffer 1 and mix\nEngage magnetic module\nDelay 7 minutes\nRemove supernatant\nDisengage magnet\nRepeat steps 8-11 with wash buffer 2 and 3\nElution solution added to sample and mixed\nDelay 5 minutes for elution\nElute added to aluminum block\n", | ||
| "reagent-setup": "\nReservoir 1: Slot 2\n\n\n\nReservoir 2: Slot 3\n\n\n", | ||
| "reagents": [ | ||
| "Omega Bio-tek" | ||
| ], | ||
| "title": "Mag-Bind\u00ae Blood & Tissue DNA HDQ 96 Kit (Fully Automated)" | ||
| } | ||