fix

protoBuilds/079db2/079db2.ot2.apiv2.py.json

@@ -1,5 +1,5 @@
 {
-    "content": "metadata = {\n    'protocolName': 'Reformatting with Custom Tube Rack',\n    'author': 'Rami Farawi <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n    [num_samp, volume, p300_mount] = get_values(  # noqa: F821\n        \"num_samp\", \"volume\", \"p300_mount\")\n\n    if not 1 <= num_samp <= 86:\n        raise Exception(\"Enter a sample number between 1-86\")\n\n    # labware\n\n    tuberacks = [ctx.load_labware('custom_24_tuberack', slot)\n                 for slot in [1, 2, 3, 4]]\n    plate = ctx.load_labware('nest_96_wellplate_2ml_deep', 6)\n    tips = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n            for slot in [8]]\n\n    # pipettes\n    p300 = ctx.load_instrument('p300_single_gen2', p300_mount, tip_racks=tips)\n\n    # mapping\n    source_wells = [tube for rack in tuberacks\n                    for row in rack.rows() for tube in row][:num_samp]\n    dest_wells = [well for row in plate.rows() for well in row][10:]\n\n    # protocol\n    ctx.comment('\\n---------------ADDING SAMPLE TO PLATE----------------\\n\\n')\n    for s, d in zip(source_wells, dest_wells):\n        p300.pick_up_tip()\n        p300.aspirate(volume, s)\n        p300.dispense(volume, d)\n        p300.drop_tip()\n        ctx.comment('\\n')\n",
+    "content": "metadata = {\n    'protocolName': 'Reformatting with Custom Tube Rack',\n    'author': 'Rami Farawi <[email protected]>',\n    'source': 'Custom Protocol Request',\n    'apiLevel': '2.13'\n}\n\n\ndef run(ctx):\n\n    [num_samp, volume, p300_mount] = get_values(  # noqa: F821\n        \"num_samp\", \"volume\", \"p300_mount\")\n\n    if not 1 <= num_samp <= 86:\n        raise Exception(\"Enter a sample number between 1-86\")\n\n    # labware\n\n    tuberacks = [ctx.load_labware('custom_24_tuberack', slot)\n                 for slot in [1, 2, 3, 4]]\n    plate = ctx.load_labware('nest_96_wellplate_2ml_deep', 6)\n    tips = [ctx.load_labware('opentrons_96_tiprack_300ul', slot)\n            for slot in [8]]\n\n    # pipettes\n    p300 = ctx.load_instrument('p300_single_gen2', p300_mount, tip_racks=tips)\n\n    # mapping\n    source_wells = [tube for rack in tuberacks\n                    for row in rack.rows() for tube in row][:num_samp]\n    dest_wells = [well for row in plate.rows() for well in row][10:]\n\n    # protocol\n    ctx.comment('\\n---------------ADDING SAMPLE TO PLATE----------------\\n\\n')\n    for s, d in zip(source_wells, dest_wells):\n        p300.pick_up_tip()\n        p300.aspirate(volume, s.bottom(z=10))\n        p300.dispense(volume, d)\n        p300.drop_tip()\n        ctx.comment('\\n')\n",
     "custom_labware_defs": [
         {
             "brand": {

protocols/079db2/079db2.ot2.apiv2.py

@@ -34,7 +34,7 @@ def run(ctx):
     ctx.comment('\n---------------ADDING SAMPLE TO PLATE----------------\n\n')
     for s, d in zip(source_wells, dest_wells):
         p300.pick_up_tip()
-        p300.aspirate(volume, s)
+        p300.aspirate(volume, s.bottom(z=10))
         p300.dispense(volume, d)
         p300.drop_tip()
         ctx.comment('\n')