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| 1 | +# Protocol Title (#3 Making Quality Control Levels in 20 ml Scintillation Vials) |
| 2 | + |
| 3 | +### Author |
| 4 | +[Opentrons](https://opentrons.com/) |
| 5 | + |
| 6 | +## Categories |
| 7 | +* Sample Prep |
| 8 | + * Standard Curve |
| 9 | + |
| 10 | +## Description |
| 11 | +This section of the README (especially the first paragraph) should grip a prospective user with the overarching purpose/flow of the protocol, but should not include fine details of the protocol steps themselves. |
| 12 | + |
| 13 | +### Labware |
| 14 | +* [OT-2 Filter Tips, 1000µL (999-00082)](https://shop.opentrons.com/opentrons-1000ul-filter-tips-1000-racks/) |
| 15 | +* [Chemglass 11-Position Block for 28 mm 20mL Scintillation Flat Bottom Vials](https://chemglass.com/blocks-for-centrifugal-vacuum-evaporators-optichem?sku=OP-6600-11) |
| 16 | + |
| 17 | +### Pipettes |
| 18 | +* [P1000 GEN2 Single Channel Pipette](https://shop.opentrons.com/single-channel-electronic-pipette-p20/) |
| 19 | + |
| 20 | +### Reagents |
| 21 | +* [Methanol](link to product not available) |
| 22 | + |
| 23 | +--- |
| 24 | + |
| 25 | +### Deck Setup |
| 26 | +* If the deck layout of a particular protocol is more or less static, it is often helpful to attach a preview of the deck layout, most descriptively generated with Labware Creator. Example: |
| 27 | + |
| 28 | + |
| 29 | +### Reagent Setup |
| 30 | +* This section can contain finer detail and images describing reagent volumes and positioning in their respective labware. Examples: |
| 31 | +* Reservoir 1: slot 5 |
| 32 | + |
| 33 | + |
| 34 | + |
| 35 | +--- |
| 36 | + |
| 37 | +### Protocol Steps |
| 38 | +1. Transfer diluent volume of MeOH into empty scintillation vials in A2 - C2. |
| 39 | +2. Perform dilutional series starting with Working Stock in A1 through C2 creating specified quality control levels. |
| 40 | + |
| 41 | + |
| 42 | +### Process |
| 43 | +1. Input your protocol parameters above. |
| 44 | +2. Download your protocol and unzip if needed. |
| 45 | +3. Upload your custom labware to the [OT App](https://opentrons.com/ot-app) by navigating to `More` > `Custom Labware` > `Add Labware`, and selecting your labware files (.json extensions) if needed. |
| 46 | +4. Upload your protocol file (.py extension) to the [OT App](https://opentrons.com/ot-app) in the `Protocol` tab. |
| 47 | +5. Set up your deck according to the deck map. |
| 48 | +6. Calibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our [support articles](https://support.opentrons.com/en/collections/1559720-guide-for-getting-started-with-the-ot-2). |
| 49 | +7. Hit 'Run'. |
| 50 | + |
| 51 | +### Additional Notes |
| 52 | +If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/). |
| 53 | + |
| 54 | +###### Internal |
| 55 | +0bc358-3 |
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