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Rename protocol, create Readme
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protocols/0bc358-3/#3 Quality Control Levels.py renamed to protocols/0bc358-3/0bc358-3.ot-2.apiv2.py

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@@ -28,7 +28,7 @@ def run(protocol: protocol_api.ProtocolContext):
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p1000 = protocol.load_instrument(
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'p1000_single_gen2', 'right', tip_racks=tips)
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def slow_withdraw(pip, well, z=0, delay_seconds=2.0):
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def slow_withdraw(pip, well, z=0, delay_seconds=0):
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pip.default_speed /= 10
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if delay_seconds > 0:
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protocol.delay(seconds=delay_seconds)

protocols/0bc358-3/README.md

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# Protocol Title (#3 Making Quality Control Levels in 20 ml Scintillation Vials)
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### Author
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[Opentrons](https://opentrons.com/)
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## Categories
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* Sample Prep
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* Standard Curve
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## Description
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This section of the README (especially the first paragraph) should grip a prospective user with the overarching purpose/flow of the protocol, but should not include fine details of the protocol steps themselves.
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### Labware
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* [OT-2 Filter Tips, 1000µL (999-00082)](https://shop.opentrons.com/opentrons-1000ul-filter-tips-1000-racks/)
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* [Chemglass 11-Position Block for 28 mm 20mL Scintillation Flat Bottom Vials](https://chemglass.com/blocks-for-centrifugal-vacuum-evaporators-optichem?sku=OP-6600-11)
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### Pipettes
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* [P1000 GEN2 Single Channel Pipette](https://shop.opentrons.com/single-channel-electronic-pipette-p20/)
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### Reagents
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* [Methanol](link to product not available)
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---
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### Deck Setup
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* If the deck layout of a particular protocol is more or less static, it is often helpful to attach a preview of the deck layout, most descriptively generated with Labware Creator. Example:
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![deck layout](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/bc-rnadvance-viral/Screen+Shot+2021-02-23+at+2.47.23+PM.png)
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### Reagent Setup
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* This section can contain finer detail and images describing reagent volumes and positioning in their respective labware. Examples:
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* Reservoir 1: slot 5
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![reservoir 1](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/1ccd23/res1_v2.png)
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---
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### Protocol Steps
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1. Transfer diluent volume of MeOH into empty scintillation vials in A2 - C2.
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2. Perform dilutional series starting with Working Stock in A1 through C2 creating specified quality control levels.
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### Process
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1. Input your protocol parameters above.
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2. Download your protocol and unzip if needed.
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3. Upload your custom labware to the [OT App](https://opentrons.com/ot-app) by navigating to `More` > `Custom Labware` > `Add Labware`, and selecting your labware files (.json extensions) if needed.
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4. Upload your protocol file (.py extension) to the [OT App](https://opentrons.com/ot-app) in the `Protocol` tab.
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5. Set up your deck according to the deck map.
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6. Calibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our [support articles](https://support.opentrons.com/en/collections/1559720-guide-for-getting-started-with-the-ot-2).
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7. Hit 'Run'.
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### Additional Notes
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If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/).
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###### Internal
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0bc358-3

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