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protocols/0bc358-2/README.md

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+# Protocol Title (#2 Making Calibrator levels in 20 ml Scintillation vials)
+
+### Author
+[Opentrons](https://opentrons.com/)
+
+## Categories
+* Sample Prep
+	* Standard Curve
+
+## Description
+This section of the README (especially the first paragraph) should grip a prospective user with the overarching purpose/flow of the protocol, but should not include fine details of the protocol steps themselves.
+
+### Labware
+* [OT-2 Filter Tips, 1000µL (999-00082)](https://shop.opentrons.com/opentrons-1000ul-filter-tips-1000-racks/)
+* [Chemglass 11-Position Block for 28 mm 20mL Scintillation Flat Bottom Vials](https://chemglass.com/blocks-for-centrifugal-vacuum-evaporators-optichem?sku=OP-6600-11)
+
+### Pipettes
+* [P1000 GEN2 Single Channel Pipette](https://shop.opentrons.com/single-channel-electronic-pipette-p20/)
+
+### Reagents
+* [Methanol](link to product not available)
+
+---
+
+### Deck Setup
+* If the deck layout of a particular protocol is more or less static, it is often helpful to attach a preview of the deck layout, most descriptively generated with Labware Creator. Example:
+![deck layout](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/bc-rnadvance-viral/Screen+Shot+2021-02-23+at+2.47.23+PM.png)
+
+### Reagent Setup
+* This section can contain finer detail and images describing reagent volumes and positioning in their respective labware. Examples:
+* Reservoir 1: slot 5
+![reservoir 1](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/1ccd23/res1_v2.png)
+
+
+---
+
+### Protocol Steps
+1. Transfer diluent volume of MeOH into empty scintillation vials in A2 - C3.
+2. Perform dilutional series starting with Working Stock in A1 through C2 creating specified calibrator levels.
+
+
+### Process
+1. Input your protocol parameters above.
+2. Download your protocol and unzip if needed.
+3. Upload your custom labware to the [OT App](https://opentrons.com/ot-app) by navigating to `More` > `Custom Labware` > `Add Labware`, and selecting your labware files (.json extensions) if needed.
+4. Upload your protocol file (.py extension) to the [OT App](https://opentrons.com/ot-app) in the `Protocol` tab.
+5. Set up your deck according to the deck map.
+6. Calibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our [support articles](https://support.opentrons.com/en/collections/1559720-guide-for-getting-started-with-the-ot-2).
+7. Hit 'Run'.
+
+### Additional Notes
+If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/).
+
+###### Internal
+0bc358-2