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{ | ||
"author": "Opentrons\nOpentrons has launched a new Protocol Library. This page won\u2019t be available after March 31st, 2024. Submit a request to add this protocol to the new library.", | ||
"author": "Opentrons", | ||
"categories": { | ||
"Nucleic Acid Extraction & Purification": [ | ||
"Viral RNA" | ||
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"description": "This protocol automates a 3-wash RNA isolation using magnetic beads and a magnetic module GEN2. Purified elutions are transferred to a 384 well plate mounted on a temperature module GEN2.\n\n\nTo purchase tips, reagents, or pipettes, please visit our online store or contact our sales team at [email protected]\n\nOpentrons OT-2\nOpentrons P300 GEN2 Multi-Channel Pipette\nOpentrons P20 GEN2 Single-Channel Pipette\nOpentrons 20uL and 200\u00b5L Filter Tips\nUSA Scientific 12-Well Reservoir, 22ml\nUSA Scientific 96-Deepwell Plate, 2.4mL #1896-2000\nCole Parmer 384-Well Plate, 50uL\n\n", | ||
"internal": "007992", | ||
"markdown": { | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n# Opentrons has launched a new Protocol Library. This page won\u2019t be available after March 31st, 2024. [Submit a request](https://docs.google.com/forms/d/e/1FAIpQLSdYYp9QCKow4nn0KlCVsMS3HX0eJ0N9O7-erajKvcpT0lWbSg/viewform) to add this protocol to the new library.\n\n", | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n\n", | ||
"categories": "* Nucleic Acid Extraction & Purification\n\t* Viral RNA\n\n", | ||
"description": "This protocol automates a 3-wash RNA isolation using magnetic beads and a magnetic module GEN2. Purified elutions are transferred to a 384 well plate mounted on a temperature module GEN2.\n\n---\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/materials.png)\n\nTo purchase tips, reagents, or pipettes, please visit our [online store](https://shop.opentrons.com/) or contact our sales team at [[email protected]](mailto:[email protected])\n\n* [Opentrons OT-2](https://shop.opentrons.com/collections/ot-2-robot/products/ot-2)\n* [Opentrons P300 GEN2 Multi-Channel Pipette](https://shop.opentrons.com/collections/ot-2-pipettes/products/8-channel-electronic-pipette)\n* [Opentrons P20 GEN2 Single-Channel Pipette](https://shop.opentrons.com/collections/ot-2-pipettes/products/single-channel-electronic-pipette)\n* [Opentrons 20uL and 200\u00b5L Filter Tips](https://shop.opentrons.com/collections/opentrons-tips)\n* [USA Scientific 12-Well Reservoir, 22ml](https://www.usascientific.com/12-channel-automation-reservoir/p/1061-8150)\n* [USA Scientific 96-Deepwell Plate, 2.4mL #1896-2000](https://www.usascientific.com/plateone-96-deep-well-2ml/p/PlateOne-96-Deep-Well-2mL)\n* Cole Parmer 384-Well Plate, 50uL\n\n---\n\n", | ||
"internal": "007992\n", | ||
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{ | ||
"author": "Opentrons\nOpentrons has launched a new Protocol Library. This page won\u2019t be available after March 31st, 2024. Submit a request to add this protocol to the new library.", | ||
"author": "Opentrons", | ||
"categories": { | ||
"Sample Prep": [ | ||
"Seeding Plate" | ||
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"description": "This protocol automates seeding plates with mammalian cells and is part of a five protocol suite. The five protocols are:\n\nProtocol 1: Seeding Plates with Mammalian Cells\nProtocol 2: DAPI Staining\nProtocol 3: Fix and DAPI Stain\nProtocol 4: Media Exchange\nProtocol 5: Primary Staining\n\nThe protocol begins with an empty reservoir for liquid waste, a 12-channel reservoir that contains what will be added to the plates, tips, eppendorf plate(s), and the P300-Multi attached to the left mount. Using up to 8 plates, the protocol will iterate through each plate and remove 50\u00b5L before adding 140\u00b5L from the reservoir. Each plate should have a designated well in the 12-well trough (i.e., slot 1 --> plate 1, slot 2 --> plate 2, etc.)\n\nIf you have any questions about this protocol, please email our Applications Engineering team at [email protected].\n\n\nTo purchase tips, reagents, or pipettes, please visit our online store or contact our sales team at [email protected]\n\nOpentrons OT-2\nOpentrons OT-2 Run App (Version 3.19.0 or later)\nOpentrons p300 Multi-Channel Pipette, GEN2\nOpentrons Tips\nNEST 12-Well Reservoir, 15mL\nNEST 1-Well Reservoir, 195mL\nEppendorf Plate\nReagents\n\n\n\nSlot 1: Opentrons Tips, note: protocol saves tip state and will prompt user to replace tips when new tips are needed.\n\nSlot 2: Eppendorf Plate (Plate 1)\n\nSlot 3: Eppendorf Plate (Plate 2)\n\nSlot 4: Eppendorf Plate (Plate 3)\n\nSlot 5: Eppendorf Plate (Plate 4)\n\nSlot 6: Eppendorf Plate (Plate 5)\n\nSlot 7: Eppendorf Plate (Plate 6)\n\nSlot 8: Eppendorf Plate (Plate 7)\n\nSlot 9: Eppendorf Plate (Plate 8)\n\nSlot 10: NEST 12-Well Reservoir, 15mL\n\nSlot 11: NEST 1-Well Reservoir, 195mL, for liquid waste\n\n\nUsing the customizations field (below), set up your protocol.\n Number of Plates: Specify the number of plates (1-8) to use.\n Aspiration/Dispense Speed: Specify the aspiration/dispense speed of the pipette (in \u00b5L/sec). Note: default rate is 43.46\u00b5L/sec.\n Aspiration Height: Specify how high (in mm) from the bottom of the well the pipette should be in the plate when aspirating.\n Dispense Height: Specify how high (in mm) from the bottom of the well the pipette should be in the plate when dispensing.\n\n", | ||
"internal": "00a214-protocol-1", | ||
"markdown": { | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n# Opentrons has launched a new Protocol Library. This page won\u2019t be available after March 31st, 2024. [Submit a request](https://docs.google.com/forms/d/e/1FAIpQLSdYYp9QCKow4nn0KlCVsMS3HX0eJ0N9O7-erajKvcpT0lWbSg/viewform) to add this protocol to the new library.\n\n", | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n\n", | ||
"categories": "* Sample Prep\n\t* Seeding Plate\n\n\n", | ||
"description": "This protocol automates seeding plates with mammalian cells and is part of a five protocol suite. The five protocols are:</br>\n</br>\nProtocol 1: [Seeding Plates with Mammalian Cells](https://develop.protocols.opentrons.com/protocol/00a214-protocol-1)</br>\nProtocol 2: [DAPI Staining](https://develop.protocols.opentrons.com/protocol/00a214-protocol-2)</br>\nProtocol 3: [Fix and DAPI Stain](https://develop.protocols.opentrons.com/protocol/00a214-protocol-3)</br>\nProtocol 4: [Media Exchange](https://develop.protocols.opentrons.com/protocol/00a214-protocol-4)</br>\nProtocol 5: [Primary Staining](https://develop.protocols.opentrons.com/protocol/00a214-protocol-5)</br>\n</br>\nThe protocol begins with an empty reservoir for liquid waste, a 12-channel reservoir that contains what will be added to the plates, tips, eppendorf plate(s), and the P300-Multi attached to the left mount. Using up to 8 plates, the protocol will iterate through each plate and remove 50\u00b5L before adding 140\u00b5L from the reservoir. Each plate should have a designated well in the 12-well trough (i.e., slot 1 --> plate 1, slot 2 --> plate 2, etc.)</br>\n</br>\nIf you have any questions about this protocol, please email our Applications Engineering team at [[email protected]](mailto:[email protected]).\n\n---\n![Materials Needed](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/materials.png)\n\nTo purchase tips, reagents, or pipettes, please visit our [online store](https://shop.opentrons.com/) or contact our sales team at [[email protected]](mailto:[email protected])\n\n* [Opentrons OT-2](https://shop.opentrons.com/collections/ot-2-robot/products/ot-2)\n* [Opentrons OT-2 Run App (Version 3.19.0 or later)](https://opentrons.com/ot-app/)\n* [Opentrons p300 Multi-Channel Pipette, GEN2](https://shop.opentrons.com/collections/ot-2-pipettes/products/8-channel-electronic-pipette)\n* [Opentrons Tips](https://shop.opentrons.com/collections/opentrons-tips)\n* [NEST 12-Well Reservoir, 15mL](https://shop.opentrons.com/collections/verified-labware/products/nest-12-well-reservoir-15-ml)\n* [NEST 1-Well Reservoir, 195mL](https://shop.opentrons.com/collections/verified-labware/products/nest-1-well-reservoir-195-ml)\n* Eppendorf Plate\n* Reagents\n\n\n\n---\n![Setup](https://s3.amazonaws.com/opentrons-protocol-library-website/custom-README-images/001-General+Headings/Setup.png)\n\nSlot 1: [Opentrons Tips](https://shop.opentrons.com/collections/opentrons-tips), note: protocol saves tip state and will prompt user to replace tips when new tips are needed.\n</br>\nSlot 2: Eppendorf Plate (Plate 1)\n</br>\nSlot 3: Eppendorf Plate (Plate 2)\n</br>\nSlot 4: Eppendorf Plate (Plate 3)\n</br>\nSlot 5: Eppendorf Plate (Plate 4)\n</br>\nSlot 6: Eppendorf Plate (Plate 5)\n</br>\nSlot 7: Eppendorf Plate (Plate 6)\n</br>\nSlot 8: Eppendorf Plate (Plate 7)\n</br>\nSlot 9: Eppendorf Plate (Plate 8)\n</br>\nSlot 10: [NEST 12-Well Reservoir, 15mL](https://shop.opentrons.com/collections/verified-labware/products/nest-12-well-reservoir-15-ml)\n</br>\nSlot 11: [NEST 1-Well Reservoir, 195mL](https://shop.opentrons.com/collections/verified-labware/products/nest-1-well-reservoir-195-ml), for liquid waste\n</br>\n</br>\n**Using the customizations field (below), set up your protocol.**\n* **Number of Plates**: Specify the number of plates (1-8) to use.\n* **Aspiration/Dispense Speed**: Specify the aspiration/dispense speed of the pipette (in \u00b5L/sec). Note: default rate is 43.46\u00b5L/sec.\n* **Aspiration Height**: Specify how high (in mm) from the bottom of the well the pipette should be in the plate when aspirating.\n* **Dispense Height**: Specify how high (in mm) from the bottom of the well the pipette should be in the plate when dispensing.\n</br>\n</br>\n\n", | ||
"internal": "00a214-protocol-1\n", | ||
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