Merge pull request #4849 from Opentrons/develop

chore(Release): 2023-08-17

data/data/fields.csv

@@ -57,6 +57,7 @@ acid_demin_yn,1
 acid_volume,1
 adapter_ligation,1
 add_ic,1
+add_transfection_mix,1
 adjust_final_vol,1
 agar_volume,1
 air_dry_time,2
@@ -100,6 +101,8 @@ assay,2
 assembly_csv,1
 assembly_transfer_vol,1
 b3_init_vol,1
+barcode_sample_plate,1
+barcode_vol,1
 barcode_well,1
 barcode_well_start,1
 bb_asp_rate_multiplier,1
@@ -268,7 +271,7 @@ csv_file_1,1
 csv_file_2,1
 csv_input,3
 csv_oil,1
-csv_samp,15
+csv_samp,16
 csv_samp1,1
 csv_samp2,1
 csv_samp3,1
@@ -309,6 +312,7 @@ dest_format,1
 dest_labware,1
 dest_lw,1
 dest_lw_def,1
+dest_plate,1
 dest_plate1,1
 dest_plate2,1
 dest_plate3,1
@@ -560,6 +564,7 @@ init_samp_vol,1
 init_temp,2
 init_time,2
 init_vol,3
+init_vol_50,1
 init_vol_bca,1
 init_vol_bsa,1
 init_vol_buff,1
@@ -712,7 +717,7 @@ lysis,1
 lysis_vol,1
 m10_mount,2
 m1k,1
-m20_mount,78
+m20_mount,79
 m300_mount,105
 m300_type,2
 m_mount,1
@@ -897,7 +902,7 @@ num_rounds,1
 num_row,1
 num_rows_a,1
 num_rows_b,1
-num_samp,69
+num_samp,70
 num_samp_p1,1
 num_samp_p2,1
 num_samp_plate1,1
@@ -922,6 +927,8 @@ num_tests,1
 num_tipracks,1
 num_tubes,2
 num_wells,2
+number_05_plates,1
+number_14_plates,1
 number_of_columns,1
 number_of_dna_samples,1
 number_of_oligo_standards,1
@@ -971,7 +978,7 @@ p1kmnt,1
 p1n,1
 p1num,1
 p20_blowout_height,1
-p20_mount,133
+p20_mount,134
 p20_multi_mount,3
 p20_rate,2
 p20_reservoir_height,1
@@ -988,7 +995,7 @@ p2num,1
 p300_gen,1
 p300_mixing_height,1
 p300_mnt,3
-p300_mount,151
+p300_mount,153
 p300_mount_1,1
 p300_multi_mount,7
 p300_rate,1
@@ -1177,6 +1184,7 @@ reagents_csv,1
 reduced_speed_limit,1
 relative_height,1
 remove_empty_racks,1
+remove_tube_vol,1
 replenish_tips_manually,1
 reps_mix,2
 res,2
@@ -1431,7 +1439,7 @@ temp_mod_s1_lname,1
 temp_mod_s4_lname,1
 temp_mod_s7_lname,1
 temp_mod_setting,3
-temp_mod_temp,1
+temp_mod_temp,2
 temp_mod_temperature,1
 temp_s1_part1,1
 temp_s1_part2,1
@@ -1522,6 +1530,7 @@ tr9,1
 track_start,1
 track_tips,2
 tracking_reset,2
+trans_mix_vol,1
 trans_vol1,1
 transfer2,1
 transfer_csv,21
@@ -1683,7 +1692,7 @@ waste,2
 waste_water_mode,1
 water_rate,1
 water_res_vol,1
-water_vol,2
+water_vol,3
 water_volume,2
 water_well,1
 wb_col,1

protoBuilds/0b97ae-protocol-2A/README.json

@@ -6,7 +6,7 @@
         ]
     },
     "deck-setup": "",
-    "description": "This is Part 2 to the QIAseq FastSelect Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nLinks:\n* Part 1: Sample Normalization\n* Part 2: QIAseq FastSelect Fragmentation",
+    "description": "This is Part 2 to the QIAseq FastSelect Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nLinks:\n Part 1: Sample Normalization\n Part 2: QIAseq FastSelect Fragmentation",
     "internal": "0b97ae-protocol-2A",
     "labware": "\nPerkin Elmer 12 Reservoir 21000 \u00b5L\nApplied Biosystems Enduraplate 96 Aluminum Block 220 \u00b5L\nOpentrons 96 Tip Rack 20 \u00b5L\nOpentrons 96 Tip Rack 300 \u00b5L\nOpentrons 96 Well Aluminum Block with Bio-Rad Well Plate 200 \u00b5L\n",
     "markdown": {

protoBuilds/0b97ae-protocol-2B/README.json

@@ -6,7 +6,7 @@
         ]
     },
     "deck-setup": "",
-    "description": "This is Part 2 to the QIAseq FastSelect 5s, 16s, 23s Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nPart 1 to this protocol is the normalization of samples.\nPart 3 to this protocol is the Extraction and Clean-Up steps.\nLinks:\n* Part 1: Sample Normalization\n* Part 2: QIAseq FastSelect 5s, 16s, 23s Fragmentation\n* Part 3: QIAseq FastSelect 5s, 16s, 23s Extraction",
+    "description": "This is Part 2 to the QIAseq FastSelect 5s, 16s, 23s Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nPart 1 to this protocol is the normalization of samples.\nPart 3 to this protocol is the Extraction and Clean-Up steps.\nLinks:\n Part 1: Sample Normalization\n Part 2: QIAseq FastSelect 5s, 16s, 23s Fragmentation\n* Part 3: QIAseq FastSelect 5s, 16s, 23s Extraction",
     "internal": "0b97ae-protocol-2B",
     "labware": "\nPerkin Elmer 12 Reservoir 21000 \u00b5L\nApplied Biosystems Enduraplate 96 Aluminum Block 220 \u00b5L\nOpentrons 96 Tip Rack 20 \u00b5L\nOpentrons 96 Tip Rack 300 \u00b5L\nOpentrons 96 Well Aluminum Block with Bio-Rad Well Plate 200 \u00b5L\n",
     "markdown": {

protoBuilds/0b97ae-protocol-3B/README.json

@@ -6,7 +6,7 @@
         ]
     },
     "deck-setup": "\nWater Reservoir (slot 2):\n    Column 1: Nuclease Free Water\n    Column 2: Binding Buffer\n    Column 3 & 4: Ethanol\n    Column 10, 11 & 12: Empty for Supernatent Removal\nDiluted RNA Plate (slot 7 Temperature Module):\n    RNA Samples Starting Plate\nReagent Plate (Slot 10):\n    Column 1: MasterMix\n    Column 2: Magentic Beads",
-    "description": "This is Part 3 to the QIAseq FastSelect 5s, 16s, 23s Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nPart 1 to this protocol is the normalization of samples.\nPart 2 to this protocol is the Fragementation.\nLinks:\n* Part 1: Sample Normalization\n* Part 2: QIAseq FastSelect 5s, 16s, 23s Fragmentation\n* Part 3: QIAseq FastSelect 5s, 16s, 23s Extraction",
+    "description": "This is Part 3 to the QIAseq FastSelect 5s, 16s, 23s Protocol. This protocol is used to perform the addition of samples with mastermix into a plate.\nPart 1 to this protocol is the normalization of samples.\nPart 2 to this protocol is the Fragementation.\nLinks:\n Part 1: Sample Normalization\n Part 2: QIAseq FastSelect 5s, 16s, 23s Fragmentation\n* Part 3: QIAseq FastSelect 5s, 16s, 23s Extraction",
     "internal": "0b97ae-protocol-3B",
     "labware": "\nPerkin Elmer 12 Reservoir 21000 \u00b5L\nApplied Biosystems Enduraplate 96 Aluminum Block 220 \u00b5L\nBio-Rad 96 Well Plate 200 \u00b5L PCR #hsp9601\nOpentrons 96 Filter Tip Rack 20 \u00b5L\nNEST 96 Deepwell Plate 2mL #503001\nOpentrons 96 Tip Rack 300 \u00b5L\nOpentrons 96 Well Aluminum Block with Bio-Rad Well Plate 200 \u00b5L\n",
     "markdown": {

protoBuilds/0b97ae/README.json

@@ -31,6 +31,6 @@
     "pipettes": "\nOpentrons P300 Single Channel Electronic Pipette (GEN2)\nOpentrons P20 Single Channel Electronic Pipette (GEN2)\n",
     "process": "\nInput your protocol parameters above.\nDownload your protocol and unzip if needed.\nUpload your custom labware to the OT App by navigating to More > Custom Labware > Add Labware, and selecting your labware files (.json extensions) if needed.\nUpload your protocol file (.py extension) to the OT App in the Protocol tab.\nSet up your deck according to the deck map.\nCalibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our support articles.\nHit \"Run\".\n",
     "protocol-steps": "\nLoad CSV into the slot above, With the Columns being RNA Concentration, Goal Concentration and Total Goal Volume\nThe Source Plate will be loaded onto the Temperature module on slot 10\nThe Dilution Plate will be loaded onto the Temperature module on slot 7\n",
-    "reagent-setup": "",
+    "reagent-setup": ".jpg)",
     "title": "QIAseq FastSelect Normalization"
 }