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Adding num of Sars-Cov-2 hits to taxonomy report #92

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mirifax opened this issue Aug 18, 2021 · 0 comments
Open

Adding num of Sars-Cov-2 hits to taxonomy report #92

mirifax opened this issue Aug 18, 2021 · 0 comments
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type:enhancement any enhancement that doesn't fit into aesthetics, bug or documentation

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@mirifax
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mirifax commented Aug 18, 2021

We noticed that there are Sars-Cov-2 hits in the Kraken report table on the taxonomy report. The Kraken2, which generates this table uses only the unaligned reads, therefore these hits should in an ideal world not be there. In order to get a sense of how many reads are actually not being aligned in the first step and therefore end up in the taxonomy table, we thought it could be good to add more information about this in the report.

This information should include:

  • number of total reads
  • number of aligned reads (to Sars-Cov-2)
  • number of unaligned reads
  • number of sars-Cov-2 hits/reads in the unaligned reads

The format could maybe be a diagram? Important is the information - how many sars-cov-2 reads are we missing in the alignment step and should that worry us?

This could be done in the markdown file which generates the report but either the number of total reads or the number of aligned reads is needed (the other numbers can be taken from the Krakenreport file). This could be done with a small bash script.

@mirifax mirifax added the type:enhancement any enhancement that doesn't fit into aesthetics, bug or documentation label Aug 18, 2021
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