From 5606c68d846f60be12209fbadab4b0492a58a62e Mon Sep 17 00:00:00 2001 From: Leighton Pritchard Date: Fri, 15 Mar 2024 08:45:27 +0000 Subject: [PATCH] add sketch introduction --- _quarto.yml | 2 +- assets/images/bing_tree.jpg | Bin 0 -> 199090 bytes assets/trees/tree_newick.nwk | 1 + index.qmd | 39 +++++++++++++++++++++++++++++------ intro.qmd | 31 ++++++++++++++++++++++++---- 5 files changed, 62 insertions(+), 11 deletions(-) create mode 100644 assets/images/bing_tree.jpg create mode 100644 assets/trees/tree_newick.nwk diff --git a/_quarto.yml b/_quarto.yml index dda3fb0..29e3915 100644 --- a/_quarto.yml +++ b/_quarto.yml @@ -24,7 +24,7 @@ book: chapters: - index.qmd -# - intro.qmd + - intro.qmd # - part: part-01_16s.qmd # chapters: # - exercise-01_16s.qmd diff --git a/assets/images/bing_tree.jpg b/assets/images/bing_tree.jpg new file mode 100644 index 0000000000000000000000000000000000000000..ee93a22ab2bf4b64d9fa912be7e8dd6ac92bcec4 GIT binary patch literal 199090 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+(((((((A:4,B:4):6,C:5):8,D:6):3,E:21):10,((F:4,G:12):14,H:8):13):13,((I:5,J:2):30,(K:11,L:11):2):17):4,M:56); diff --git a/index.qmd b/index.qmd index 7e9edb0..b6599a8 100644 --- a/index.qmd +++ b/index.qmd @@ -1,9 +1,36 @@ -# Preface {.unnumbered} +# Preface to {{< var ay >}} BM211 Workshop 5: Phylogenetic Trees {.unnumbered} -This is a Quarto book. +Welcome to the BM211 (Introduction to Microbiology) workshop on phylogenetic trees, for {{< var ay >}}. -To learn more about Quarto books visit . +This year is the first presentation of the workshop material in this format, and we would be very grateful to hear feedback [by email](mailto:{{< var admin.email >}}) or through the [GitHub repository Issues page]({{< var github.issues >}}). + +## Overview + +This workshop asks you to work through some computational exercises to gain some practice visualising and working with phylogenetic trees. + +::: { .callout-note } +Phylogenetic trees are typically constructed _after_ sequence alignment has been performed. You learned about the fundamentals of (pairwise) sequence alignment in BM214 Workshop 2 - [slides](https://classes.myplace.strath.ac.uk/mod/resource/view.php?id=1956504), [workshop](https://sipbs-compbiol.github.io/BM214-Workshop-2/). You may find it useful to refresh your memory about pairwise alignment. +::: + +::: { .callout-tip } +We also provide an ["`R` playground"](playground.qmd) if you would like to get further practice interacting with and plotting data in `R`. +::: + +::: { .callout-warning title="Important Note" } +There is new material in this workshop that is not covered in lectures, and this material is examinable. Please take care to reach the text in the expandable callout boxes, as well as that for the workshop activities, to be sure you have understood the topic and obtain full value from the exercises. +::: + +## Learning Objectives + +By the end of this workshop, students will be able to: + +[**FILL IN TEXT HERE**] + +## Assessment + +The workshop is not formally assessed although, as noted above, all the material contained in the workshop is examinable. [**FILL IN TEXT HERE**] + +::: { .callout-important } +There are three formative exercises, one for each exercise in the workshop, and they can be found on the workshop [MyPlace page]({{< var myplace.bm211 >}}). You should complete these as part of the workshop. +::: -```{r} -1 + 1 -``` diff --git a/intro.qmd b/intro.qmd index e8cd16c..ad432c5 100644 --- a/intro.qmd +++ b/intro.qmd @@ -1,9 +1,32 @@ # Introduction -This is a book created from markdown and executable code. +## Working with phylogenetic trees -See @knuth84 for additional discussion of literate programming. +When you use a software tool to build, or you otherwise acquire, phylogenetic tree data it will be in one of a number of file formats, such as: -```{r} -1 + 1 +- [`newick`](https://en.wikipedia.org/wiki/Newick_format) +- [`NEXUS`](https://en.wikipedia.org/wiki/Nexus_file) +- [`phylip`](https://en.wikipedia.org/wiki/PHYLIP) + +These files can be cryptic to read, as a human. For example, the first `newick` format file we will work with has these contents: + +```text +(((((((A:4,B:4):6,C:5):8,D:6):3,E:21):10,((F:4,G:12):14,H:8):13):13,((I:5,J:2):30,(K:11,L:11):2):17):4,M:56); ``` + +It is difficult, as a human, to read a file like this and understand the structure of the tree it describes, so we almost always use computational tools to open these files and visualise the trees they represent. + +![A genAI representation of "phylogenetic tree as a real tree."](assets/images/bing_tree.jpg){#fig-bing-tree width=80%} + +### Why we are using `R` + +A number of software packages are available to visualise, edit, and refine phylogenetic tree files for reports and publications. Some of these can be downloaded and run on your own computer, such as: + +- [`figtree`]() _this is the one I use most often - LP_ +- [`dendroscope`]() + +but the [interactive Tree of Life (iTol)](https://itol.embl.de/) service allows you, once you register and sign in, to edit trees directly in the browser. Traditionally, phylogenetic trees have been visualised using independent software tools like these. + +In this workshop we will be using `R` with the `ggtree` package to visualise and interpret trees. The `R` software ecosystem is a very powerful tool for computational biology and bioinformatics work, encompassing sequence analysis and genomics, transcriptomic and evolutionary analyses, with excellent visualisation capabilities. As `R` is also a programming language, large parts of the analysis can be automated and made reproducible, which is an advantage over independent tools, and a factor in the growing popularity of this approach. Basic competence and skills in `R` are in high demand in academia and industry. + +