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# SciJava Ops Flim: A fluorescence lifetime analysis library for SciJava Ops. | ||
# SciJava Ops FLIM: A fluorescence lifetime analysis library for SciJava Ops. | ||
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This library provides a bridge between SciJava Ops and Flim. | ||
For Maven projects, this library can be used by adding to your `pom.xml`: | ||
SciJava Ops FLIM is a collection of FLIM analysis ops based on [FLIMLib](https://github.com/flimlib/flimlib). It extends the single-transient fitting functions in FLIMLib to dataset-level fitting ops. Currently supported fitting ops include: RLD, MLA, Global, Phasor, and single-component Bayesian. | ||
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```java | ||
<dependency> | ||
<groupId>org.scijava</groupId> | ||
<artifactId>scijava-ops-flim</artifactId> | ||
<version>0-SNAPSHOT</version> | ||
</dependency> | ||
Besides curve fitting, SciJava Ops FLIM also provides a variety of pre-processing options such as pixel binning, intensity thresholding, ROI masking as well as post-processing utility ops for e.g. calculating τ<sub>m</sub> (mean lifetime), A<sub>i</sub>% (fractional contribution) and pseudocoloring the result with LUT. | ||
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# Example usage | ||
Open [test2.sdt](test_files/test2.sdt) in [Fiji](https://fiji.github.io/). Execute in [Script Editor](http://imagej.github.io/Using_the_Script_Editor) as Groovy: | ||
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```groovy | ||
#@ UIService ui | ||
#@ OpEnvironment op | ||
#@ ImgPlus img | ||
// set up parameters | ||
import org.scijava.ops.flim.FitParams | ||
param = new FitParams() | ||
param.transMap = img; // input 3-dimensional (x, y, t) dataset | ||
param.xInc= 0.040 // time difference between bins (ns) | ||
param.ltAxis = 2 // time bins lay along axis #2 | ||
// op call | ||
fittedImg = op.unary("flim.fitLMA").input(param).apply().paramMap | ||
// display each parameter | ||
zImg = op.ternary("transform.hyperSliceView").input(fittedImg, param.ltAxis, 0L).apply() | ||
AImg = op.ternary("transform.hyperSliceView").input(fittedImg, param.ltAxis, 1L).apply() | ||
tauImg = op.ternary("transform.hyperSliceView").input(fittedImg, param.ltAxis, 2L).apply() | ||
ui.show("z", zImg) | ||
ui.show("A", AImg) | ||
ui.show("tau", tauImg) | ||
``` | ||
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After running this script, the output shown below can be seen by first running the script, and then brightness and contrast (Ctrl + Shift + C, select "Set" and bound the contrast to each image's corresponding range below). | ||
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(z in [-1, 1], A in [0, 4], tau in[0, 3]): | ||
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![example output](images/example%20z.png)![example output](images/example%20A.png)![example output](images/example%20tau.png) | ||
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See more examples in [Demo.ipynb](notebooks/Demo.ipynb) and [groovy.md](groovy.md). | ||
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# Using from a Java project | ||
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To depend on SciJava Ops FLIM, copy the following to your `pom.xml`: | ||
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```xml | ||
<properties> | ||
<scijava-ops-flim.version>0-SNAPSHOT</scijava-ops-flim.version> | ||
</properties> | ||
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<dependencies> | ||
<dependency> | ||
<groupId>org.scijava</groupId> | ||
<artifactId>scijava-ops-flim</artifactId> | ||
<version>${scijava-ops-flim.version}</version> | ||
</dependency> | ||
</dependencies> | ||
``` | ||
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# See also | ||
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- [FLIMLib](https://github.com/flimlib/flimlib): Curve fitting library for FLIM | ||
- [Debug tutorial](https://github.com/flimlib/flimlib/wiki/Debugging) | ||
- [FLIMJ Ops](https://github.com/flimlib/flimj-ops): ImageJ Ops for accessing FLIM | ||
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# Citation | ||
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Comming soon... |