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ERROR with tabix_selector in PREPARE_REPEATS subworkflow #22

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BethYates opened this issue Jul 2, 2024 · 3 comments
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ERROR with tabix_selector in PREPARE_REPEATS subworkflow #22

BethYates opened this issue Jul 2, 2024 · 3 comments
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@BethYates
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Description of the bug

The pipeline fails on the PREPARE_REPEATS:TABIX_TABIX_TBI process. The calculation in the PREPARE_REPEATS subworkflow that works out if tbi_and_csi indexing can be used or if only_csi indexing can be used does not appear to be working when the pipeline is downloading the Corroboree frog Pseudophryne corroboree genome, GCA_028390025.1 https://www.ebi.ac.uk/ena/browser/view/GCA_028390025.1

Command used and terminal output

nextflow run sanger-tol/insdcdownload --assembly_accession GCA_028390025.1 --assembly_name aPseCor3.hap2 -r 1.1.0 -profile sanger,singularity

ERROR ~ Error executing process > 'SANGERTOL_INSDCDOWNLOAD:INSDCDOWNLOAD:PREPARE_REPEATS:TABIX_TABIX_TBI (GCA_028390025.1.masked.ncbi)'

Caused by:
  Process `SANGERTOL_INSDCDOWNLOAD:INSDCDOWNLOAD:PREPARE_REPEATS:TABIX_TABIX_TBI (GCA_028390025.1.masked.ncbi)` terminated with an error exit status (1)

Command executed:

  tabix --preset bed GCA_028390025.1.masked.ncbi.bed.gz
  
  cat <<-END_VERSIONS > versions.yml
  "SANGERTOL_INSDCDOWNLOAD:INSDCDOWNLOAD:PREPARE_REPEATS:TABIX_TABIX_TBI":
      tabix: $(echo $(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*$//')
  END_VERSIONS

Command exit status:
  1

Command output:
  (empty)

Command error:
  [E::hts_idx_check_range] Region 536869515..536870967 cannot be stored in a tbi index. Try using a csi index
  tbx_index_build failed: GCA_028390025.1.masked.ncbi.bed.gz

Relevant files

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System information

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@BethYates BethYates added the bug Something isn't working label Jul 2, 2024
@tkchafin
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tkchafin commented Jul 3, 2024

I think this was fixed in #16 -- is there a reason you need 1.1.0 or could you try re-running with 2.0.0?

@BethYates
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Re-running with 2.0.0 works - thanks.

@BethYates
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Fix already made in newer version

@github-project-automation github-project-automation bot moved this from Todo to Done in Genome After Party Jul 11, 2024
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