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When taking images of the bead channel, one time some of the settings got mixed up which led to images of rather bad quality. They had bad contrast/dynamic range and were rather blurry. Nevertheless I was able to generate reasonable traction force maps using Fiji, while pytraction seemed to struggle in these cases.
For the Fiji pipeline I use rolling ball background subtraction after stack alignment which I think might have helped.
Here is the link to some example images and the outputs I got. For each image it contains following files:
filename
description
comment
Magnitude map ...
magnitude map generated with FiJi pipeline
pos_...
cropped images and roi used for pytraction pipeline; pdf file of traction force map generated by pytraction
wc = with cell; woc = without cell
..._RAW
raw images
ch00 = phase contrast; ch01 = beads
The text was updated successfully, but these errors were encountered:
When taking images of the bead channel, one time some of the settings got mixed up which led to images of rather bad quality. They had bad contrast/dynamic range and were rather blurry. Nevertheless I was able to generate reasonable traction force maps using Fiji, while pytraction seemed to struggle in these cases.
For the Fiji pipeline I use rolling ball background subtraction after stack alignment which I think might have helped.
Here is the link to some example images and the outputs I got. For each image it contains following files:
pdf file of traction force map generated by pytraction
The text was updated successfully, but these errors were encountered: