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In low contrast/underexposed images FiJi seems to detect forces more robustly #56

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l-kaplan opened this issue Aug 5, 2021 · 0 comments
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l-kaplan commented Aug 5, 2021

When taking images of the bead channel, one time some of the settings got mixed up which led to images of rather bad quality. They had bad contrast/dynamic range and were rather blurry. Nevertheless I was able to generate reasonable traction force maps using Fiji, while pytraction seemed to struggle in these cases.
For the Fiji pipeline I use rolling ball background subtraction after stack alignment which I think might have helped.

Here is the link to some example images and the outputs I got. For each image it contains following files:

filename description comment
Magnitude map ... magnitude map generated with FiJi pipeline
pos_... cropped images and roi used for pytraction pipeline;
pdf file of traction force map generated by pytraction
wc = with cell; woc = without cell
..._RAW raw images ch00 = phase contrast; ch01 = beads
@rg314 rg314 self-assigned this Aug 9, 2021
@rg314 rg314 added the enhancement New feature or request label Aug 9, 2021
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