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Using a 5' PAM for enumeration #27

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ericmalekos opened this issue Apr 3, 2024 · 0 comments
Open

Using a 5' PAM for enumeration #27

ericmalekos opened this issue Apr 3, 2024 · 0 comments

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@ericmalekos
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Thank you for the great tool.
Is there anyway to enumerate off-targets under the assumption that that the PAM is 5' to the protospacer?
For Cas12A for example the targets are 5' - PAM (TTTV) - protospacer - 3'
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@ericmalekos