diff --git a/instrumentation/opentelemetry-instrumentation-grpc/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements-1.txt
index 7618e99dfa..278f27cb9f 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements-1.txt
@@ -1,6 +1,6 @@
 asgiref==3.8.1
 Deprecated==1.2.14
-grpcio==1.63.0
+grpcio==1.66.2
 iniconfig==2.0.0
 packaging==24.0
 pluggy==1.5.0
diff --git a/tox.ini b/tox.ini
index eddd6c61ea..9d79a11f8b 100644
--- a/tox.ini
+++ b/tox.ini
@@ -265,7 +265,7 @@ envlist =
     ; The numbers at the end of the environment names
     ; below mean these dependencies are being used:
     ; 0: grpcio==1.62.0
-    ; 1: grpcio==1.63.0
+    ; 1: grpcio==1.66.2
     py3{8,9,10,11,12}-test-instrumentation-grpc-{0,1}
     lint-instrumentation-grpc