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FluoviewReader: Timepoints split over multiple files not recognized as single dataset #4223

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dgault opened this issue Aug 15, 2024 · 1 comment

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@dgault
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dgault commented Aug 15, 2024

Issue was raised in forum thread https://forum.image.sc/t/how-to-open-video-in-matlab-using-bioformats-plugin-when-the-source-data-is-split-into-multiple-tif-files/99909/6

Sample files reproducing the issue in Bio-Formats 7.3.1 were provided and are available at inbox/imagesc-99909

The sample data file consists of 2 TIFF files, with the timepoints split across the 2 files. The first file contains 3984 timepoints and the second file contains 324 timepoints.

As the dimensions of the 2 files are different it is not possible to use a pattern file or the FileStitcher to concatenate the 2 files together. Using an OME-TIFF companion file may be an option but would require manual modification of the OME-XML file. Fixing this at the reader level to allow for these files to be read as a single dataset may be the best option.

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This issue has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/how-to-open-video-in-matlab-using-bioformats-plugin-when-the-source-data-is-split-into-multiple-tif-files/99909/10

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