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I've noticed that in some cases, sequences that have no significant alignment to a reference achieve higher scores (and much lower e-values) than those that align perfectly.
In particular, this occurs with the NC_000913.3 reference sequence when aligning the following reads in a file called test.fa
The first read has very few matches to the reference, but BlastFrost reports and e-value of 1e-427, with the underlying score being 334, whereas the second read matches exactly, but gets and e-value of 1e-427 and score of 136.
I am using commit d425ac6 (BlastFrost) and 82adeedd5eb1007fdfae2324b339ae32c1c128d3 (bifrost).
Any guidance would be greatly appreciated.
Best regards,
Harun
The text was updated successfully, but these errors were encountered:
hmusta
changed the title
Non-matching sequences achieving high scores
Non-matching sequences achieving much high scores than exact matching sequences
Dec 9, 2021
I've noticed that in some cases, sequences that have no significant alignment to a reference achieve higher scores (and much lower e-values) than those that align perfectly.
In particular, this occurs with the
NC_000913.3
reference sequence when aligning the following reads in a file calledtest.fa
The first read has very few matches to the reference, but BlastFrost reports and e-value of 1e-427, with the underlying score being 334, whereas the second read matches exactly, but gets and e-value of 1e-427 and score of 136.
I built the graph using the command
and aligned with
I am using commit d425ac6 (BlastFrost) and 82adeedd5eb1007fdfae2324b339ae32c1c128d3 (bifrost).
Any guidance would be greatly appreciated.
Best regards,
Harun
The text was updated successfully, but these errors were encountered: