This directory contains m-functions for reading and writing files used in electron microscopy and 3D reconstruction. The file formats those used by the IMAGIC software package (Image Science GmbH; EMAN and Frealign are public-domain programs that also use this format), the MRC program library, and the Digital Micrograph (Gatan, Inc.) file format. These functions were written on what published information we could find, and work for our limited purposes; they are not supported by the software authors at all.
The functions generally assume that little-endian files are being read by a little-endian machine. However, some functions might still work with big-endian machines such as PowerPC, as they were originally written on a Mac computer.
The original code was developed by Fred Sigworth and Liguo Wang at Yale University and provided through mathworks.
Problems when reading MRC files prompted me to do some changes to ReadMRC().
Niko Komin LCSB/University of Luxembourg 2016
ReadImagic
Loads part or all of the data in an Imagic file pair into a matlab 3-d array. This function also returns a data structure with part of the information from the header.
WriteImagic
Writes an entire matlab 3-d array as an Imagic file with float32 data type.
ReadImagicHeader MakeImagicHeader WriteImagicHeader
Create, read or write a datastructure that contains all the information in an Imagic header. The structure (a struct of arrays) allows manipulation of header information. For writing very large datafiles, the header file can be constructed and written out separately by these functions.
ReadDM3
Read a file generated by Digital Micrograph, version 3. These files have a large tree structure, and the code inside this function can be modified to return any field or fields of this structure. At present just the pixel size and number of pixels is returned along with the raw image.
ReadMRC
Read part or all of an MRC-format file (2d or 3d) into a Matlab array; also retrieves information about the image size.
WriteMRC
Write an entire 3d Matlab array into an MRC-format file
WriteMRCHeader
Write the header of an MRC-format file, allowing the user to write the rest of the file directly with the fwrite function.