diff --git a/tests/component/test_stream_readers_di.py b/tests/component/test_stream_readers_di.py
index ee84de3d..007e7836 100644
--- a/tests/component/test_stream_readers_di.py
+++ b/tests/component/test_stream_readers_di.py
@@ -9,10 +9,7 @@
 import nidaqmx
 import nidaqmx.system
 from nidaqmx.constants import LineGrouping
-from nidaqmx.stream_readers import (
-    DigitalMultiChannelReader,
-    DigitalSingleChannelReader,
-)
+from nidaqmx.stream_readers import DigitalMultiChannelReader, DigitalSingleChannelReader
 from nidaqmx.utils import flatten_channel_string
 
 
@@ -400,6 +397,25 @@ def test___digital_multi_channel_reader___read_one_sample_multi_line___returns_v
     )
 
 
+def test___digital_multi_channel_reader___read_one_sample_multi_line_jagged___returns_valid_samples(
+    di_multi_channel_port_uint32_task: nidaqmx.Task,
+) -> None:
+    reader = DigitalMultiChannelReader(di_multi_channel_port_uint32_task.in_stream)
+    num_channels = di_multi_channel_port_uint32_task.number_of_channels
+    samples_to_read = 256
+    sample = numpy.full((num_channels, 32), False, dtype=numpy.bool_)
+
+    data = [
+        _read_and_copy(reader.read_one_sample_multi_line, sample) for _ in range(samples_to_read)
+    ]
+
+    assert [
+        [_bool_array_to_int(sample[chan, :]) for chan in range(num_channels)] for sample in data
+    ] == _get_expected_digital_port_data_sample_major(
+        di_multi_channel_port_uint32_task, samples_to_read
+    )
+
+
 def test___digital_multi_channel_reader___read_one_sample_multi_line_with_wrong_dtype___raises_error_with_correct_dtype(
     di_multi_channel_multi_line_task: nidaqmx.Task,
 ) -> None:
diff --git a/tests/component/test_stream_writers_do.py b/tests/component/test_stream_writers_do.py
index 4a832270..a4a2d952 100644
--- a/tests/component/test_stream_writers_do.py
+++ b/tests/component/test_stream_writers_do.py
@@ -171,6 +171,19 @@ def di_port1_loopback_task(
     return task
 
 
+@pytest.fixture
+def di_port2_loopback_task(
+    generate_task: Callable[[], nidaqmx.Task], real_x_series_device: nidaqmx.system.Device
+) -> nidaqmx.Task:
+    task = generate_task()
+    task.di_channels.add_di_chan(
+        real_x_series_device.di_ports[2].name,
+        line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
+    )
+    _start_di_task(task)
+    return task
+
+
 @pytest.fixture
 def di_multi_channel_port_loopback_task(
     generate_task: Callable[[], nidaqmx.Task], real_x_series_device: nidaqmx.system.Device
@@ -431,6 +444,34 @@ def test___digital_multi_channel_writer___write_one_sample_multi_line___updates_
     assert di_multi_line_loopback_task.read() == datum
 
 
+def test___digital_multi_channel_writer___write_one_sample_multi_line_jagged___updates_output(
+    di_port0_loopback_task: nidaqmx.Task,
+    di_port1_loopback_task: nidaqmx.Task,
+    di_port2_loopback_task: nidaqmx.Task,
+    generate_task: Callable[[], nidaqmx.Task],
+    real_x_series_device: nidaqmx.system.Device,
+) -> None:
+    task = generate_task()
+    for port in real_x_series_device.do_ports:
+        task.do_channels.add_do_chan(
+            port.name,
+            line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
+        )
+    _start_do_task(task, is_port=True, num_chans=task.number_of_channels)
+    writer = DigitalMultiChannelWriter(task.out_stream)
+    num_channels = task.number_of_channels
+    samples_to_write = 0xA5
+
+    # "sweep" up to the final value, the only one we'll validate
+    for datum in _get_digital_data(num_channels * 32, samples_to_write):
+        data_to_write = _int_to_bool_array(num_channels * 32, datum).reshape((num_channels, 32))
+        writer.write_one_sample_multi_line(data_to_write)
+
+    assert di_port0_loopback_task.read() == datum & 0xFFFFFFFF
+    assert di_port1_loopback_task.read() == (datum >> 32) & 0xFF
+    assert di_port2_loopback_task.read() == (datum >> 64) & 0xFF
+
+
 def test___digital_multi_channel_writer___write_one_sample_multi_line_with_wrong_dtype___raises_error_with_correct_dtype(
     do_multi_channel_multi_line_task: nidaqmx.Task,
 ) -> None: