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Issue with SPADES2/Kmer length #44

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chiroptera19 opened this issue Apr 6, 2021 · 1 comment
Open

Issue with SPADES2/Kmer length #44

chiroptera19 opened this issue Apr 6, 2021 · 1 comment

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@chiroptera19
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Hello! I absolutely love the ORP and have run it successfully with a lot of my reads. However, I'm running into quite the head scratcher for reads that are 75 bp long or less. The program documentation says you can specify your SPADES2_KMER=INT length, which I have done in the following lines:

MAKEDIR := $(dir $(firstword $(MAKEFILE_LIST)))
DIR := ${CURDIR}
CPU=16
BUSCO_THREADS=${CPU}
MEM=110
TRINITY_KMER=25
SPADES1_KMER=55
SPADES2_KMER=35
TRANSABYSS_KMER=32
RCORR := ${shell which rcorrector}

My reads are exactly 75 bp long, but changing the SPADES2_KMER flag does not resolve the issue. I still receive the following error:

IT LOOKS LIKE YOUR READS ARE NOT AT LEAST 75 BP LONG,
PLEASE EDIT YOUR COMMAND USING THE SPADES2_KMER=INT FLAGS,
SETTING THE ASSEMBLY KMER LENGTH LESS THAN YOUR READ LENGTH

/bin/bash: line 8: shell: command not found

I found a discussion about this on the github from 2019: #17

Was this ever resolved/addressed? How can I get ORP to run for reads that are 75bp or less? Hoping to hear back!

@macmanes
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sorry, yes this has been updated in the most recent release.

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