Goals.lyx

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\begin_layout Chapter
Research aims
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coś tu na początek jeszcze napisać
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The unique zinc binding hook from the 
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 protein inspired me to ask questions: how many 
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 and 
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 are structurally described.
 The first goal of the research was to provide a survey of intermolecularly
 bound Zn(II) ions available in the 
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.
 The idea was to algorithmically filter for the physiological candidates
 of 
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, followed by the manual assessement of the validity of the filtered structures.
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The second goal, an expansion of the first idea, was to provide freely,
 and easily-available reso urce of all 
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 deposited in the 
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.
 To do I have developed InterMetalDB, using 
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 and 
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.
 The service, and its open 
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 are available online.
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The third aim (which inspired formulation of two previous ideas) of my doctoral
 project was to perform biophysical characterization of the zinc hook domain
 of the human protein 
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.
 
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 protein, occurs in the 
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 core complex in every domain of life—a key complex involved in 
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gls{dna}
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 damage response.
 In eukaryotes 
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 core complex is stabilized and regulated by an additional interaction partner—
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 (or 
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 in 
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), forming thus the 
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 complex.
 The conserved zinc hook domain in 
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 is an example of unique 
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, where the dimerization is strictly dependent on the Zn(II) ion co-cordination
 by two 
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 protomers.
 The formation of 
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 dimer is essential for the proper functioning of the 
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, which is directly related to the functioning of the whole 
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(
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) complex.
 
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coś tu jeszcze napisać
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Finally, the last aim of the research was to investigate the potential detriment
al effect of the mismetallation of the zinc hook from the 
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.
 This has been done by investigation of the effect of Hg(II) and Ag(I) on
 the zinc hook from the 
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.
 The choice of the 
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 to be the investigation target was the fact that this model is so far best
 characterized model of the zinc hook available.
 
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