#Intro

GFF files that are the output of taking RNA-seq data through a Trinity/Augustus pipeline.

#Crude method overview Libraries were made from tissues of adult virgin males and females collected 2-5 days post eclosion. Carcass and germline tissue libraries were prepped and sequence separately and sequenced in 4-plex using Illumina barcodes. Further methods are available in Rogers et al. 2014.

#Pipeline

We are working on describing our methods via explicit scripts/examples here. That is a work in progress.

To build the GFF files, FASTQ files from all sexes, tissues, and replicates were concatenated before running through our pipeline.

#Citation

Users should cite Rogers et al. 2014 "Sex-biased expression in the Drosophila melanogaster group" available as a preprint in ArXiv.