diff --git a/src/libraries/System.Private.CoreLib/src/System/Diagnostics/Tracing/EventSource.cs b/src/libraries/System.Private.CoreLib/src/System/Diagnostics/Tracing/EventSource.cs
index 2761e0037e20a9..feabfae3e18835 100644
--- a/src/libraries/System.Private.CoreLib/src/System/Diagnostics/Tracing/EventSource.cs
+++ b/src/libraries/System.Private.CoreLib/src/System/Diagnostics/Tracing/EventSource.cs
@@ -3896,10 +3896,14 @@ internal static void InitializeDefaultEventSources()
                 return;
             }
 
-// NOTE: this define is being used inconsistently. Most places mean just EventPipe support, but then a few places use
-// it to mean other aspects of tracing such as these EventSources.
+            // NOTE: this define is being used inconsistently. Most places mean just EventPipe support, but then a few places use
+            // it to mean other aspects of tracing such as these EventSources.
 #if FEATURE_PERFTRACING
             _ = NativeRuntimeEventSource.Log;
+            // ThreadPoolWorkQueue uses FrameworkEventSource during ThreadPool initialization. If that is the first time
+            // the FrameworkEventSource is being used, creating it on-demand will acquire the EventListener lock which can deadlock.
+            // See https://github.com/dotnet/runtime/issues/126591. We avoid that by pre-creating the FrameworkEventSource here.
+            _ = FrameworkEventSource.Log;
 #if !TARGET_BROWSER
             _ = RuntimeEventSource.Log;
 #endif