@@ -14,37 +14,39 @@ The tools
1414.. contents ::
1515 :local:
1616
17- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
18- | tool | type | input files | main output file(s) | application |
19- +================================+==================+===================================+============================================+===================================================================================+
20- | :doc:`tools/multiBamSummary ` | data integration | 2 or more BAM | interval-based table of values | perform cross-sample analyses of read counts --> plotCorrelation, plotPCA |
21- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
22- | :doc:`tools/multiBigwigSummary ` | data integration | 2 or more bigWig | interval-based table of values | perform cross-sample analyses of genome-wide scores --> plotCorrelation, plotPCA |
23- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
24- | :doc:`tools/plotCorrelation ` | visualization | bam/multiBigwigSummary output | clustered heatmap | visualize the Pearson/Spearman correlation |
25- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
26- | :doc:`tools/plotPCA ` | visualization | bam/multiBigwigSummary output | 2 PCA plots | visualize the principal component analysis |
27- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
28- | :doc:`tools/plotFingerprint ` | QC | 2 BAM | 1 diagnostic plot | assess enrichment strength of a ChIP sample |
29- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
30- | :doc:`tools/computeGCBias ` | QC | 1 BAM | 2 diagnostic plots | calculate the exp. and obs. GC distribution of reads |
31- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
32- | :doc:`tools/correctGCBias ` | QC | 1 BAM, output from computeGCbias | 1 GC-corrected BAM | obtain a BAM file with reads distributed according to the genome’s GC content |
33- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
34- | :doc:`tools/bamCoverage ` | normalization | BAM | bedGraph or bigWig | obtain the normalized read coverage of a single BAM file |
35- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
36- | :doc:`tools/bamCompare ` | normalization | 2 BAM | bedGraph or bigWig | normalize 2 files to each other (e.g. log2ratio, difference) |
37- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
38- | :doc:`tools/computeMatrix ` | data integration | 1 or more bigWig, 1 or more BED | zipped file for plotHeatmap or plotProfile | compute the values needed for heatmaps and summary plots |
39- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
40- | :doc:`tools/plotHeatmap ` | visualization | computeMatrix output | heatmap of read coverages | visualize the read coverages for genomic regions |
41- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
42- | :doc:`tools/plotProfile ` | visualization | computeMatrix output | summary plot (“meta-profile”) | visualize the average read coverages over a group of genomic regions |
43- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
44- | :doc:`tools/plotCoverage ` | visualization | 1 or more bam | 2 diagnostic plots | visualize the average read coverages over sampled genomic positions |
45- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
46- | :doc:`tools/bamPEFragmentSize ` | information | 1 BAM | text with paired-end fragment length | obtain the average fragment length from paired ends |
47- +--------------------------------+------------------+-----------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
17+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
18+ | tool | type | input files | main output file(s) | application |
19+ +================================+==================+=====================================+============================================+===================================================================================+
20+ | :doc:`tools/multiBamSummary ` | data integration | 2 or more BAM | interval-based table of values | perform cross-sample analyses of read counts --> plotCorrelation, plotPCA |
21+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
22+ | :doc:`tools/multiBigwigSummary ` | data integration | 2 or more bigWig | interval-based table of values | perform cross-sample analyses of genome-wide scores --> plotCorrelation, plotPCA |
23+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
24+ | :doc:`tools/plotCorrelation ` | visualization | bam/multiBigwigSummary output | clustered heatmap | visualize the Pearson/Spearman correlation |
25+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
26+ | :doc:`tools/plotPCA ` | visualization | bam/multiBigwigSummary output | 2 PCA plots | visualize the principal component analysis |
27+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
28+ | :doc:`tools/plotFingerprint ` | QC | 2 BAM | 1 diagnostic plot | assess enrichment strength of a ChIP sample |
29+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
30+ | :doc:`tools/computeGCBias ` | QC | 1 BAM | 2 diagnostic plots | calculate the exp. and obs. GC distribution of reads |
31+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
32+ | :doc:`tools/correctGCBias ` | QC | 1 BAM, output from computeGCbias | 1 GC-corrected BAM | obtain a BAM file with reads distributed according to the genome’s GC content |
33+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
34+ | :doc:`tools/bamCoverage ` | normalization | BAM | bedGraph or bigWig | obtain the normalized read coverage of a single BAM file |
35+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
36+ | :doc:`tools/bamCompare ` | normalization | 2 BAM | bedGraph or bigWig | normalize 2 files to each other (e.g. log2ratio, difference) |
37+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
38+ | :doc:`tools/computeMatrix ` | data integration | 1 or more bigWig, 1 or more BED | zipped file for plotHeatmap or plotProfile | compute the values needed for heatmaps and summary plots |
39+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
40+ | :doc:`tools/plotHeatmap ` | visualization | computeMatrix output | heatmap of read coverages | visualize the read coverages for genomic regions |
41+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
42+ | :doc:`tools/plotProfile ` | visualization | computeMatrix output | summary plot (“meta-profile”) | visualize the average read coverages over a group of genomic regions |
43+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
44+ | :doc:`tools/plotCoverage ` | visualization | 1 or more BAM | 2 diagnostic plots | visualize the average read coverages over sampled genomic positions |
45+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
46+ | :doc:`tools/bamPEFragmentSize ` | information | 1 BAM | text with paired-end fragment length | obtain the average fragment length from paired ends |
47+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
48+ | :doc:`tools/plotEnrichment ` | visualization | 1 or more BAM and 1 or more BED/GTF | A diagnostic plot | plots the fraction of alignments overlapping the given features |
49+ +--------------------------------+------------------+-------------------------------------+--------------------------------------------+-----------------------------------------------------------------------------------+
4850
4951General principles
5052^^^^^^^^^^^^^^^^^^
@@ -144,3 +146,5 @@ Heatmaps and summary plots
144146""""""""""""""""""""""""
145147:doc: `tools/plotProfile `
146148""""""""""""""""""""""""
149+ :doc: `tools/plotEnrichment `
150+ """""""""""""""""""""""""""
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