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To detect SSU: There is not a lot of conserved short-range secondary structure at the 3' end of SSU. Either use a CM including all of V10 and the helix it sits on, or HMMs (the same ones used in ITSx?).
brendanf
changed the title
Detect 5' end of SSU
Detect 3' end of SSU
Jun 16, 2020
This would be helpful for studies using primers that include enough SSU to be useful.
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