DADA2 ITS trimming question

[UniAlberta]

For analyzing ITS amplicon data, in filterAndTrim function "out <- filterAndTrim(cutFs, filtFs, cutRs, filtRs, maxN = 0, maxEE = c(2, 2), truncQ = 2, minLen = 50, rm.phix = TRUE, compress = TRUE, multithread = TRUE)", I see that there is no truncLen for forward and reverse. But 16S has that argument. Can you please explain that?

Thanks

[benjjneb]

There is high natural variability in the lengths of the ITS region. Therefore, imposing a fixed truncation length will either exclude real ITS sequences that are shorter than the length and/or prevent merging of reads from longer ITS sequences by truncating them before they overlap.

[UniAlberta]

Thank you so much for the explanation. Also, I have another question. I used the DADA2 ITS pipeline in 2022 for my fungal data analysis but unfortunately, I forgot to record the version I used. Can you help find the version for that year so I can put it in my paper?

[benjjneb]

I used the DADA2 ITS pipeline in 2022 for my fungal data analysis but unfortunately, I forgot to record the version I used. Can you help find the version for that year so I can put it in my paper?

It could have been multiple versions depended on when in the year you installed it, and what outlet it came from. I'm not comfortable just stating a version that it could have been.

[UniAlberta]

Thanks! I used this tutorial so I`ll refer to v 1.8.
https://benjjneb.github.io/dada2/ITS_workflow.html