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I have 4C experiments to process. Naturally, I have MILLIONS of anchor zone reads. And the speed of intersection creation is unbearable slow. Each of the replicates contains almost 15 million reads in total. 4 hours have passed already, bedtools intersect has only processed 3.8 million reads, and the file is growing VERY, VERY slowly.
The commandline is:
I have PE reads so I don't like the idea to convert it all to bedgraph and then find intersections in 5 seconds.
bedtools uses only 1 CPU core, although computer has a plenty of free cores.
Ubuntu 22.04 x64, SSD Samsung 980 Pro 1TB.
I don't need in 'true' intersection, I need in complete list of unmodified reads/alignments that are intersected in replicates files to obtain the BAM file with intersected reads to process it further with featureCounts. Are there alternatives to bedtools for this task?
The text was updated successfully, but these errors were encountered:
I have 4C experiments to process. Naturally, I have MILLIONS of anchor zone reads. And the speed of intersection creation is unbearable slow. Each of the replicates contains almost 15 million reads in total. 4 hours have passed already, bedtools intersect has only processed 3.8 million reads, and the file is growing VERY, VERY slowly.
The commandline is:
I have PE reads so I don't like the idea to convert it all to bedgraph and then find intersections in 5 seconds.
bedtools uses only 1 CPU core, although computer has a plenty of free cores.
Ubuntu 22.04 x64, SSD Samsung 980 Pro 1TB.
I don't need in 'true' intersection, I need in complete list of unmodified reads/alignments that are intersected in replicates files to obtain the BAM file with intersected reads to process it further with featureCounts. Are there alternatives to bedtools for this task?
The text was updated successfully, but these errors were encountered: