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Hi,
there are many tools (bbmerge.sh from BBmap, VSEARCH, USEARCH, ...) which can look for some overlap between members of a FASTA/Q read pair but is there a tool which can use already mapped pair memebers to a common reference and merge them? I have the .fastq.gz files still around so it could maybe just act on position-sorted SAM/BAM and poke through the synced read pairs in R1 and R2 files and merge them accordingly (replace Ns with a nucleotide from the other mate, eventually prefer higher QUAL)?
Hi,
there are many tools (bbmerge.sh from BBmap, VSEARCH, USEARCH, ...) which can look for some overlap between members of a FASTA/Q read pair but is there a tool which can use already mapped pair memebers to a common reference and merge them? I have the
.fastq.gz
files still around so it could maybe just act on position-sorted SAM/BAM and poke through the synced read pairs inR1
andR2
files and merge them accordingly (replaceN
s with a nucleotide from the other mate, eventually prefer higherQUAL
)?https://sourceforge.net/projects/bbmap/
https://drive5.com/usearch/manual/merge_pair.html
https://github.com/jsh58/NGmerge
https://cme.h-its.org/exelixis/web/software/pear/
https://gitlab.com/german.tischler/biobambam2/-/blob/master/src/programs/bamtofastq.1
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